The eclipse period of Escherichia coli

U von Freiesleben; M A Krekling; F G Hansen; A Løbner-Olesen

doi:10.1093/emboj/19.22.6240

The eclipse period of Escherichia coli

U von Freiesleben, M A Krekling, F G Hansen, A Løbner-Olesen

74 Citationer (Scopus)

Abstract

The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation. The SeqA protein was absolutely required for the eclipse, and DnaA titration studies suggested that the SeqA protein prevented the binding of multiple DnaA molecules on oriC (initial complex formation). No correlation between the amount of SeqA and eclipse length was revealed, but increased SeqA levels affected chromosome partitioning and/or cell division. This was corroborated further by an aberrant nucleoid distribution in SeqA-deficient cells. We suggest that the SeqA protein's role in maintaining the eclipse is tied to a function in chromosome organization.

Originalsprog	Engelsk
Tidsskrift	E M B O Journal
Vol/bind	19
Udgave nummer	22
Sider (fra-til)	6240-8
Antal sider	9
ISSN	0261-4189
DOI	https://doi.org/10.1093/emboj/19.22.6240
Status	Udgivet - 15 nov. 2000

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10.1093/emboj/19.22.6240

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@article{29532ea9cf0b47999ab021305a399877,

title = "The eclipse period of Escherichia coli",

abstract = "The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation. The SeqA protein was absolutely required for the eclipse, and DnaA titration studies suggested that the SeqA protein prevented the binding of multiple DnaA molecules on oriC (initial complex formation). No correlation between the amount of SeqA and eclipse length was revealed, but increased SeqA levels affected chromosome partitioning and/or cell division. This was corroborated further by an aberrant nucleoid distribution in SeqA-deficient cells. We suggest that the SeqA protein's role in maintaining the eclipse is tied to a function in chromosome organization.",

keywords = "Bacterial Outer Membrane Proteins, Bacterial Proteins/genetics, Base Sequence, Cell Cycle, Cell Division, Chromosomes, Bacterial/metabolism, DNA Primers/genetics, DNA Replication, DNA-Binding Proteins/metabolism, Escherichia coli/cytology, Escherichia coli Proteins, Kinetics, Mutation, Origin Recognition Complex, Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism, Transcription Factors, Viral Proteins",

author = "{von Freiesleben}, U and Krekling, {M A} and Hansen, {F G} and A L{\o}bner-Olesen",

year = "2000",

month = nov,

day = "15",

doi = "10.1093/emboj/19.22.6240",

language = "English",

volume = "19",

pages = "6240--8",

journal = "E M B O Journal",

issn = "0261-4189",

publisher = "Wiley-Blackwell",

number = "22",

}

TY - JOUR

T1 - The eclipse period of Escherichia coli

AU - von Freiesleben, U

AU - Krekling, M A

AU - Hansen, F G

AU - Løbner-Olesen, A

PY - 2000/11/15

Y1 - 2000/11/15

N2 - The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation. The SeqA protein was absolutely required for the eclipse, and DnaA titration studies suggested that the SeqA protein prevented the binding of multiple DnaA molecules on oriC (initial complex formation). No correlation between the amount of SeqA and eclipse length was revealed, but increased SeqA levels affected chromosome partitioning and/or cell division. This was corroborated further by an aberrant nucleoid distribution in SeqA-deficient cells. We suggest that the SeqA protein's role in maintaining the eclipse is tied to a function in chromosome organization.

AB - The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation. The SeqA protein was absolutely required for the eclipse, and DnaA titration studies suggested that the SeqA protein prevented the binding of multiple DnaA molecules on oriC (initial complex formation). No correlation between the amount of SeqA and eclipse length was revealed, but increased SeqA levels affected chromosome partitioning and/or cell division. This was corroborated further by an aberrant nucleoid distribution in SeqA-deficient cells. We suggest that the SeqA protein's role in maintaining the eclipse is tied to a function in chromosome organization.

KW - Bacterial Outer Membrane Proteins

KW - Bacterial Proteins/genetics

KW - Base Sequence

KW - Cell Cycle

KW - Cell Division

KW - Chromosomes, Bacterial/metabolism

KW - DNA Primers/genetics

KW - DNA Replication

KW - DNA-Binding Proteins/metabolism

KW - Escherichia coli/cytology

KW - Escherichia coli Proteins

KW - Kinetics

KW - Mutation

KW - Origin Recognition Complex

KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism

KW - Transcription Factors

KW - Viral Proteins

U2 - 10.1093/emboj/19.22.6240

DO - 10.1093/emboj/19.22.6240

M3 - Journal article

C2 - 11080169

SN - 0261-4189

VL - 19

SP - 6240

EP - 6248

JO - E M B O Journal

JF - E M B O Journal

IS - 22

ER -

The eclipse period of Escherichia coli

Abstract

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