The Drosophila Microtubule-Associated Protein Mars Stabilizes Mitotic Spindles by Crosslinking Microtubules through Its N-Terminal Region

Gang Zhang; Hamze Beati; Jakob Nilsson; Andreas Wodarz

doi:10.1371/journal.pone.0060596

The Drosophila Microtubule-Associated Protein Mars Stabilizes Mitotic Spindles by Crosslinking Microtubules through Its N-Terminal Region

Gang Zhang, Hamze Beati, Jakob Nilsson, Andreas Wodarz

The NNF Center for Protein Research

9 Citationer (Scopus)

Abstract

Correct segregation of genetic material relies on proper assembly and maintenance of the mitotic spindle. How the highly dynamic microtubules (MTs) are maintained in stable mitotic spindles is a key question to be answered. Motor and non-motor microtubule associated proteins (MAPs) have been reported to stabilize the dynamic spindle through crosslinking adjacent MTs. Mars, a novel MAP, is essential for the early development of Drosophila embryos. Previous studies showed that Mars is required for maintaining an intact mitotic spindle but did not provide a molecular mechanism for this function. Here we show that Mars is able to stabilize the mitotic spindle in vivo. Both in vivo and in vitro data reveal that the N-terminal region of Mars functions in the stabilization of the mitotic spindle by crosslinking adjacent MTs.

Originalsprog	Engelsk
Tidsskrift	P L o S One
Vol/bind	8
Udgave nummer	4
Sider (fra-til)	e60596
Antal sider	10
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0060596
Status	Udgivet - 4 apr. 2013

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10.1371/journal.pone.0060596

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0060596

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AU - Wodarz, Andreas

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AB - Correct segregation of genetic material relies on proper assembly and maintenance of the mitotic spindle. How the highly dynamic microtubules (MTs) are maintained in stable mitotic spindles is a key question to be answered. Motor and non-motor microtubule associated proteins (MAPs) have been reported to stabilize the dynamic spindle through crosslinking adjacent MTs. Mars, a novel MAP, is essential for the early development of Drosophila embryos. Previous studies showed that Mars is required for maintaining an intact mitotic spindle but did not provide a molecular mechanism for this function. Here we show that Mars is able to stabilize the mitotic spindle in vivo. Both in vivo and in vitro data reveal that the N-terminal region of Mars functions in the stabilization of the mitotic spindle by crosslinking adjacent MTs.

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The Drosophila Microtubule-Associated Protein Mars Stabilizes Mitotic Spindles by Crosslinking Microtubules through Its N-Terminal Region

Abstract

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