The differential plasma proteome of obese and overweight individuals undergoing a nutritional weight loss and maintenance intervention

TY - JOUR

T1 - The differential plasma proteome of obese and overweight individuals undergoing a nutritional weight loss and maintenance intervention

AU - Oller Moreno, Sergio

AU - Cominetti, Ornella

AU - Núñez Galindo, Antonio

AU - Irincheeva, Irina

AU - Corthésy, John

AU - Astrup, Arne

AU - Saris, Wim H M

AU - Hager, Jörg

AU - Kussmann, Martin

AU - Dayon, Loïc

N1 - CURIS 2018 NEXS 037

PY - 2018/1

Y1 - 2018/1

N2 - Purpose: The nutritional intervention program “DiOGenes” focuses on how obesity can be prevented and treated from a dietary perspective. We generated differential plasma proteome profiles in the DiOGenes cohort to identify proteins associated with weight loss and maintenance and explore their relation to body mass index, fat mass, insulin resistance, and sensitivity. Experimental design: Relative protein quantification was obtained at baseline and after combined weight loss/maintenance phases using isobaric tagging and MS/MS. A Welch t-test determined proteins differentially present after intervention. Protein relationships with clinical variables were explored using univariate linear models, considering collection center, gender and age as confounding factors. Results: Four hundred and seventy three subjects were measured at baseline and end of the intervention; 39 proteins were longitudinally differential. Proteins with largest changes were sex hormone-binding globulin, adiponectin, C-reactive protein, calprotectin, serum amyloid A, and proteoglycan 4 (PRG4), whose association with obesity and weight loss is known. We identified new putative biomarkers for weight loss/maintenance. Correlation between PRG4 and proline-rich acidic protein 1 variation and Matsuda insulin sensitivity increment was showed. Conclusion and clinical relevance: MS-based proteomic analysis of a large cohort of non-diabetic overweight and obese individuals concomitantly identified known and novel proteins associated with weight loss and maintenance.

AB - Purpose: The nutritional intervention program “DiOGenes” focuses on how obesity can be prevented and treated from a dietary perspective. We generated differential plasma proteome profiles in the DiOGenes cohort to identify proteins associated with weight loss and maintenance and explore their relation to body mass index, fat mass, insulin resistance, and sensitivity. Experimental design: Relative protein quantification was obtained at baseline and after combined weight loss/maintenance phases using isobaric tagging and MS/MS. A Welch t-test determined proteins differentially present after intervention. Protein relationships with clinical variables were explored using univariate linear models, considering collection center, gender and age as confounding factors. Results: Four hundred and seventy three subjects were measured at baseline and end of the intervention; 39 proteins were longitudinally differential. Proteins with largest changes were sex hormone-binding globulin, adiponectin, C-reactive protein, calprotectin, serum amyloid A, and proteoglycan 4 (PRG4), whose association with obesity and weight loss is known. We identified new putative biomarkers for weight loss/maintenance. Correlation between PRG4 and proline-rich acidic protein 1 variation and Matsuda insulin sensitivity increment was showed. Conclusion and clinical relevance: MS-based proteomic analysis of a large cohort of non-diabetic overweight and obese individuals concomitantly identified known and novel proteins associated with weight loss and maintenance.

KW - Biomarker

KW - Diabetes

KW - Large-scale study

KW - Mass spectrometry

KW - Obesity

KW - Proteomics

U2 - 10.1002/prca.201600150

DO - 10.1002/prca.201600150

M3 - Journal article

C2 - 28371297

SN - 1862-8346

VL - 12

JO - Proteomics - Clinical Applications

JF - Proteomics - Clinical Applications

IS - 1

M1 - 1600150

ER -