@article{829260f0b0a011ddb538000ea68e967b,
title = "The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation",
abstract = "TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. At least two distinct pathways exist for down-regulation of the TCR. One pathway is activated following TCR ligation and is dependent on tyrosine phosphorylation. The other pathway is dependent on protein kinase C (PKC)-mediated activation of the CD3 gamma di-leucine-based receptor-sorting motif. Previous studies have failed to demonstrate a connection between ligand- and PKC-induced TCR down-regulation. Thus, although an apparent paradox, the dogma has been that ligand- and PKC-induced TCR down-regulations are not interrelated. By analyses of a newly developed CD3 gamma-negative T cell variant, freshly isolated and PHA-activated PBMC, and a mouse T cell line, we challenged this dogma and demonstrate in this work that PKC activation and the CD3 gamma di-leucine-based motif are indeed required for efficient ligand-induced TCR down-regulation.",
author = "{von Essen}, Marina and Charlotte Menn{\'e} and Nielsen, {Bodil L} and Lauritsen, {Jens Peter H} and Jes Dietrich and Andersen, {Peter S} and Klaus Karjalainen and Niels {\O}dum and Carsten Geisler",
note = "Keywords: Amino Acid Motifs; Amino Acid Sequence; Animals; Antigens, CD3; Cell Line; Cells, Cultured; Down-Regulation; Gene Deletion; Humans; Jurkat Cells; Kinetics; Leucine; Ligands; Mice; Molecular Sequence Data; Protein Kinase C; Receptor-CD3 Complex, Antigen, T-Cell; Sequence Homology; T-Lymphocytes",
year = "2002",
language = "English",
volume = "168",
pages = "4519--23",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",
}