The association between measurements of antimicrobial use and resistance in the faeces microbiota of finisher batches

TY - JOUR

T1 - The association between measurements of antimicrobial use and resistance in the faeces microbiota of finisher batches

AU - Andersen, V D

AU - DE Knegt, L V

AU - Munk, P

AU - Jensen, M S

AU - Agersø, Y

AU - Aarestrup, F M

AU - Vigre, H

PY - 2017/10/1

Y1 - 2017/10/1

N2 - The objectives were to present three approaches for calculating antimicrobial (AM) use in pigs that take into account the rearing period and rearing site, and to study the association between these measurements and phenotypical resistance and abundance of resistance genes in faeces samples from 10 finisher batches. The AM use was calculated relative to the rearing period of the batches as (i) 'Finisher Unit Exposure' at unit level, (ii) 'Lifetime Exposure' at batch level and (iii) 'Herd Exposure' at herd level. A significant effect on the occurrence of tetracycline resistance measured by cultivation was identified for Lifetime Exposure for the AM class: tetracycline. Furthermore, for Lifetime Exposure for the AM classes: macrolide, broad-spectrum penicillin, sulfonamide and tetracycline use as well as Herd Unit Exposure for the AM classes: aminoglycoside, lincosamide and tetracycline use, a significant effect was observed on the occurrence of genes coding for the AM resistance classes: aminoglycoside, lincosamide, macrolide, β-lactam, sulfonamide and tetracycline. No effect was observed for Finisher Unit Exposure. Overall, the study shows that Lifetime Exposure is an efficient measurement of AM use in finisher batches, and has a significant effect on the occurrence of resistance, measured either by cultivation or metagenomics.

AB - The objectives were to present three approaches for calculating antimicrobial (AM) use in pigs that take into account the rearing period and rearing site, and to study the association between these measurements and phenotypical resistance and abundance of resistance genes in faeces samples from 10 finisher batches. The AM use was calculated relative to the rearing period of the batches as (i) 'Finisher Unit Exposure' at unit level, (ii) 'Lifetime Exposure' at batch level and (iii) 'Herd Exposure' at herd level. A significant effect on the occurrence of tetracycline resistance measured by cultivation was identified for Lifetime Exposure for the AM class: tetracycline. Furthermore, for Lifetime Exposure for the AM classes: macrolide, broad-spectrum penicillin, sulfonamide and tetracycline use as well as Herd Unit Exposure for the AM classes: aminoglycoside, lincosamide and tetracycline use, a significant effect was observed on the occurrence of genes coding for the AM resistance classes: aminoglycoside, lincosamide, macrolide, β-lactam, sulfonamide and tetracycline. No effect was observed for Finisher Unit Exposure. Overall, the study shows that Lifetime Exposure is an efficient measurement of AM use in finisher batches, and has a significant effect on the occurrence of resistance, measured either by cultivation or metagenomics.

KW - Animals

KW - Anti-Infective Agents/administration & dosage

KW - Denmark

KW - Drug Resistance, Microbial

KW - Feces/microbiology

KW - Microbial Sensitivity Tests/veterinary

KW - Microbiota/drug effects

KW - Sus scrofa/microbiology

U2 - 10.1017/s0950268817001285

DO - 10.1017/s0950268817001285

M3 - Journal article

C2 - 28651652

SN - 0950-2688

VL - 145

SP - 2827

EP - 2837

JO - Epidemiology and Infection

JF - Epidemiology and Infection

IS - 13

ER -