The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

TY - JOUR

T1 - The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

AU - von Malek, Bernadette

AU - van der Graaff, Eric

AU - Schneitz, Kay

AU - Keller, Beat

PY - 2002/11

Y1 - 2002/11

N2 - The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.

AB - The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.

KW - Acetates

KW - Amino Acid Sequence

KW - Arabidopsis

KW - Chromosome Mapping

KW - Chromosomes, Plant

KW - Cyclopentanes

KW - Fertility

KW - Genetic Complementation Test

KW - Intramolecular Oxidoreductases

KW - Molecular Sequence Data

KW - Mutation

KW - Oxylipins

KW - Sequence Homology, Amino Acid

U2 - 10.1007/s00425-002-0906-2

DO - 10.1007/s00425-002-0906-2

M3 - Journal article

C2 - 12430030

SN - 0032-0943

VL - 216

SP - 187

EP - 192

JO - Planta Medica

JF - Planta Medica

IS - 1

ER -