Temporal pattern of mutations in the knockdown resistance (kdr) gene of Aedes aegypti mosquitoes sampled from Southern Taiwan

TY - JOUR

T1 - Temporal pattern of mutations in the knockdown resistance (kdr) gene of Aedes aegypti mosquitoes sampled from Southern Taiwan

AU - Biduda, Sandrine

AU - Lin, Chia-Hsien

AU - Saleh, Fatma

AU - Konradsen, Flemming

AU - Hansson, Helle

AU - Schiøler, Karin L

AU - Alifrangis, Michael

PY - 2019

Y1 - 2019

N2 - Aedes mosquitoes are the principal dengue vector in Taiwan, where the use of insecticides is a key element in the national control strategy. However, control efforts are constrained by the development of resistance to most insecticides, including pyrethroids. In this study, mutations in the voltage-gated sodium channel (VGSC) gene resulting in knockdown resistance (kdr) were examined in Aedes aegypti. Fragments of the VGSC gene were polymerase chain reaction (PCR)-amplified followed by restriction fragment length polymorphism analysis in samples from various settings in Southern Taiwan covering dry and wet seasons from 2013 to 2015. Three kdr mutations were identified: V1023G, D1794Y, and F1534C, with observed frequencies of 0.36, 0.55, and 0.33, respectively, in the dry season of 2013–2014. Exploring for temporal changes, the most important observation was the 1534C allele frequency increment in the following season to 0.60 (P < 0.05). This study suggests that continued insecticide pressure is driving the mutational changes, although the selection is ambiguous in the mosquito population.

AB - Aedes mosquitoes are the principal dengue vector in Taiwan, where the use of insecticides is a key element in the national control strategy. However, control efforts are constrained by the development of resistance to most insecticides, including pyrethroids. In this study, mutations in the voltage-gated sodium channel (VGSC) gene resulting in knockdown resistance (kdr) were examined in Aedes aegypti. Fragments of the VGSC gene were polymerase chain reaction (PCR)-amplified followed by restriction fragment length polymorphism analysis in samples from various settings in Southern Taiwan covering dry and wet seasons from 2013 to 2015. Three kdr mutations were identified: V1023G, D1794Y, and F1534C, with observed frequencies of 0.36, 0.55, and 0.33, respectively, in the dry season of 2013–2014. Exploring for temporal changes, the most important observation was the 1534C allele frequency increment in the following season to 0.60 (P < 0.05). This study suggests that continued insecticide pressure is driving the mutational changes, although the selection is ambiguous in the mosquito population.

U2 - 10.4269/ajtmh.19-0289

DO - 10.4269/ajtmh.19-0289

M3 - Journal article

C2 - 31516108

SN - 0002-9637

VL - 101

SP - 973

EP - 975

JO - Journal. National Malaria Society

JF - Journal. National Malaria Society

IS - 5

ER -