Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads

Linn S. Mamsen; Emil H. Ernst; Rehannah Borup; Agnete Larsen; Rasmus H. Olesen; Erik Ernst; Richard A. Anderson; Stine G. Kristensen; Claus Y. Andersen

doi:10.1038/s41598-017-15931-3

Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads

Linn S. Mamsen^*, Emil H. Ernst, Rehannah Borup, Agnete Larsen, Rasmus H. Olesen, Erik Ernst, Richard A. Anderson, Stine G. Kristensen, Claus Y. Andersen

^*Corresponding author af dette arbejde

Institut for Klinisk Medicin

16 Citationer (Scopus)

54 Downloads (Pure)

Abstract

The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries. Gene expression was confirmed by IHC for GAGE, SOX9, AMH, CYP17A1, LIN28, WNT2B, ETV5 and GLI1. Gene expression was not associated with the maternal smoking habits. Collectively, a precise temporal determination of changes in expression of key genes involved in human sex-differentiation is defined, with identification of new genes of potential importance.

Originalsprog	Engelsk
Artikelnummer	15961
Tidsskrift	Scientific Reports
Vol/bind	7
Antal sider	16
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-017-15931-3
Status	Udgivet - 2017

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10.1038/s41598-017-15931-3Licens: CC BY

Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonadsForlagets udgivne version, 3,1 MBLicens: CC BY

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title = "Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads",

abstract = "The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries. Gene expression was confirmed by IHC for GAGE, SOX9, AMH, CYP17A1, LIN28, WNT2B, ETV5 and GLI1. Gene expression was not associated with the maternal smoking habits. Collectively, a precise temporal determination of changes in expression of key genes involved in human sex-differentiation is defined, with identification of new genes of potential importance.",

author = "Mamsen, {Linn S.} and Ernst, {Emil H.} and Rehannah Borup and Agnete Larsen and Olesen, {Rasmus H.} and Erik Ernst and Anderson, {Richard A.} and Kristensen, {Stine G.} and Andersen, {Claus Y.}",

year = "2017",

doi = "10.1038/s41598-017-15931-3",

language = "English",

volume = "7",

journal = "Scientific Reports",

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T1 - Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads

AU - Mamsen, Linn S.

AU - Ernst, Emil H.

AU - Borup, Rehannah

AU - Larsen, Agnete

AU - Olesen, Rasmus H.

AU - Ernst, Erik

AU - Anderson, Richard A.

AU - Kristensen, Stine G.

AU - Andersen, Claus Y.

PY - 2017

Y1 - 2017

N2 - The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries. Gene expression was confirmed by IHC for GAGE, SOX9, AMH, CYP17A1, LIN28, WNT2B, ETV5 and GLI1. Gene expression was not associated with the maternal smoking habits. Collectively, a precise temporal determination of changes in expression of key genes involved in human sex-differentiation is defined, with identification of new genes of potential importance.

AB - The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries. Gene expression was confirmed by IHC for GAGE, SOX9, AMH, CYP17A1, LIN28, WNT2B, ETV5 and GLI1. Gene expression was not associated with the maternal smoking habits. Collectively, a precise temporal determination of changes in expression of key genes involved in human sex-differentiation is defined, with identification of new genes of potential importance.

U2 - 10.1038/s41598-017-15931-3

DO - 10.1038/s41598-017-15931-3

M3 - Journal article

C2 - 29162857

AN - SCOPUS:85034744592

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 15961

ER -

Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads

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