Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri i.p. vaccination

Martin Kristian Raida, Kurt Buchmann

    Abstract

    The immune response in rainbow trout against a bacterin of Yersinia ruckeri, a bacterial pathogen causing enteric red mouth disease (ERM), was investigated at 5, 15 and 25° C. Rainbow trout were immunized by i.p. injection of a Y. ruckeri (serotype O1) water based bacterin and compared to control groups injected with phosphate buffered saline (PBS). Blood and tissue samples (spleen and head-kidney) were taken for subsequent analysis using solid phase enzyme-linked immunosorbent assay (ELISA) and real-time PCR (RQ-PCR), respectively. The up-regulation of cytokine genes was generally faster and higher at high water temperature with major expression at 25° C. The pro-inflammatory cytokine IL-1ß and INF¿ was significantly up-regulated in all immunized groups whereas the cytokine IL-10 was merely up-regulated in fish kept at 15 and 25° C. The gene encoding the C5a (anaphylatoxin) receptor was expressed at a significantly increased level in both head-kidney and spleen of immunized fish. The secreted IgM encoding gene was significantly up-regulated in the head-kidney of immunized trout reared at 25° C, and a positive correlation (r: 0.663) was found between gene expression of secreted IgM in the head-kidney and Y. ruckeri specific antibodies in plasma measured by ELISA. However, no regulation of the teleost specific immunoglobulin IgT, which was generally expressed at a much lower level than IgM, could be detected. The study indicated that both innate and specific adaptive immune response factors are highly temperature-dependent in rainbow trout.
    OriginalsprogEngelsk
    TitelIkke angivet
    RedaktørerKurt Buchmann
    Antal sider1
    ForlagFaculty of Life Sciences, Department of Veterinary Pathobiology
    Publikationsdato2007
    Sider16
    StatusUdgivet - 2007
    BegivenhedAbildgaard Workshop: COST Action 867 Training School. Welfare of Farmed Fish - Nutrition, Breeding and Diseases - Frederiksberg, Danmark
    Varighed: 11 apr. 200712 apr. 2007

    Konference

    KonferenceAbildgaard Workshop: COST Action 867 Training School. Welfare of Farmed Fish - Nutrition, Breeding and Diseases
    Land/OmrådeDanmark
    ByFrederiksberg
    Periode11/04/200712/04/2007

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