Abstract
The immune response in rainbow trout against a bacterin of Yersinia ruckeri, a bacterial pathogen causing enteric red mouth disease (ERM), was investigated at 5, 15 and 25° C. Rainbow trout were immunized by i.p. injection of a Y. ruckeri (serotype O1) water based bacterin and compared to control groups injected with phosphate buffered saline (PBS). Blood and tissue samples (spleen and head-kidney) were taken for subsequent analysis using solid phase enzyme-linked immunosorbent assay (ELISA) and real-time PCR (RQ-PCR), respectively. The up-regulation of cytokine genes was generally faster and higher at high water temperature with major expression at 25° C. The pro-inflammatory cytokine IL-1ß and INF¿ was significantly up-regulated in all immunized groups whereas the cytokine IL-10 was merely up-regulated in fish kept at 15 and 25° C. The gene encoding the C5a (anaphylatoxin) receptor was expressed at a significantly increased level in both head-kidney and spleen of immunized fish. The secreted IgM encoding gene was significantly up-regulated in the head-kidney of immunized trout reared at 25° C, and a positive correlation (r: 0.663) was found between gene expression of secreted IgM in the head-kidney and Y. ruckeri specific antibodies in plasma measured by ELISA. However, no regulation of the teleost specific immunoglobulin IgT, which was generally expressed at a much lower level than IgM, could be detected. The study indicated that both innate and specific adaptive immune response factors are highly temperature-dependent in rainbow trout.
Originalsprog | Engelsk |
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Titel | Ikke angivet |
Redaktører | Kurt Buchmann |
Antal sider | 1 |
Forlag | Faculty of Life Sciences, Department of Veterinary Pathobiology |
Publikationsdato | 2007 |
Sider | 16 |
Status | Udgivet - 2007 |
Begivenhed | Abildgaard Workshop: COST Action 867 Training School. Welfare of Farmed Fish - Nutrition, Breeding and Diseases - Frederiksberg, Danmark Varighed: 11 apr. 2007 → 12 apr. 2007 |
Konference
Konference | Abildgaard Workshop: COST Action 867 Training School. Welfare of Farmed Fish - Nutrition, Breeding and Diseases |
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Land/Område | Danmark |
By | Frederiksberg |
Periode | 11/04/2007 → 12/04/2007 |