Temperature-controlled structure and kinetics of ripple phases in one- and two-component supported lipid bilayers

Thomas Kaasgaard; Chad Leidy; John H. Crowe; Ole G. Mouritsen; Kent Jørgensen

doi:10.1016/S0006-3495(03)74479-8

Temperature-controlled structure and kinetics of ripple phases in one- and two-component supported lipid bilayers

Thomas Kaasgaard, Chad Leidy, John H. Crowe, Ole G. Mouritsen, Kent Jørgensen^*

^*Corresponding author af dette arbejde

110 Citationer (Scopus)

Abstract

Temperature-controlled atomic force microscopy (AFM) has been used to visualize and study the structure and kinetics of ripple phases in one-component dipalmitoylphosphatidylcholine (DPPC) and two-component dimyristoylphosphatidylcholine-distearoylphosphatidylcholine (DMPC-DSPC) lipid bilayers. The lipid bilayers are mica-supported double bilayers in which ripple-phase formation occurs in the top bilayer. In one-component DPPC lipid bilayers, the stable and metastable ripple phases were observed. In addition, a third ripple structure with approximately twice the wavelength of the metastable ripples was seen. From height profiles of the AFM images, estimates of the amplitudes of the different ripple phases are reported. To elucidate the processes of ripple formation and disappearance, a ripple-phase DPPC lipid bilayer was taken through the pretransition in the cooling and the heating direction and the disappearance and formation of ripples was visualized. It was found that both the disappearance and formation of ripples take place virtually one ripple at a time, thereby demonstrating the highly anisotropic nature of the ripple phase. Furthermore, when a two-component DMPC-DSPC mixture was heated from the ripple phase and into the ripple-phase/fluid-phase coexistence temperature region, the AFM images revealed that several dynamic properties of the ripple phase are important for the melting behavior of the lipid mixture. Onset of melting is observed at grain boundaries between different ripple types and different ripple orientations, and the longer-wavelength metastable ripple phase melts before the shorter-wavelength stable ripple phase. Moreover, it was observed that the ripple phase favors domain growth along the ripple direction and is responsible for creating straight-edged domains with 60° and 120° angles, as reported previously.

Originalsprog	Engelsk
Tidsskrift	Biophysical Journal
Vol/bind	85
Udgave nummer	1
Sider (fra-til)	350-360
Antal sider	11
ISSN	0006-3495
DOI	https://doi.org/10.1016/S0006-3495(03)74479-8
Status	Udgivet - 1 jul. 2003

Adgang til dokumentet

10.1016/S0006-3495(03)74479-8

Andre filer og links

Link to publication in Scopus

Citationsformater

@article{37cf7acd8310447e8afd450f11668f48,

title = "Temperature-controlled structure and kinetics of ripple phases in one- and two-component supported lipid bilayers",

abstract = "Temperature-controlled atomic force microscopy (AFM) has been used to visualize and study the structure and kinetics of ripple phases in one-component dipalmitoylphosphatidylcholine (DPPC) and two-component dimyristoylphosphatidylcholine-distearoylphosphatidylcholine (DMPC-DSPC) lipid bilayers. The lipid bilayers are mica-supported double bilayers in which ripple-phase formation occurs in the top bilayer. In one-component DPPC lipid bilayers, the stable and metastable ripple phases were observed. In addition, a third ripple structure with approximately twice the wavelength of the metastable ripples was seen. From height profiles of the AFM images, estimates of the amplitudes of the different ripple phases are reported. To elucidate the processes of ripple formation and disappearance, a ripple-phase DPPC lipid bilayer was taken through the pretransition in the cooling and the heating direction and the disappearance and formation of ripples was visualized. It was found that both the disappearance and formation of ripples take place virtually one ripple at a time, thereby demonstrating the highly anisotropic nature of the ripple phase. Furthermore, when a two-component DMPC-DSPC mixture was heated from the ripple phase and into the ripple-phase/fluid-phase coexistence temperature region, the AFM images revealed that several dynamic properties of the ripple phase are important for the melting behavior of the lipid mixture. Onset of melting is observed at grain boundaries between different ripple types and different ripple orientations, and the longer-wavelength metastable ripple phase melts before the shorter-wavelength stable ripple phase. Moreover, it was observed that the ripple phase favors domain growth along the ripple direction and is responsible for creating straight-edged domains with 60° and 120° angles, as reported previously.",

author = "Thomas Kaasgaard and Chad Leidy and Crowe, {John H.} and Mouritsen, {Ole G.} and Kent J{\o}rgensen",

year = "2003",

month = jul,

day = "1",

doi = "10.1016/S0006-3495(03)74479-8",

language = "English",

volume = "85",

pages = "350--360",

journal = "Biophysical Society. Annual Meeting. Abstracts",

issn = "0523-6800",

publisher = "Biophysical Society",

number = "1",

}

TY - JOUR

T1 - Temperature-controlled structure and kinetics of ripple phases in one- and two-component supported lipid bilayers

AU - Kaasgaard, Thomas

AU - Leidy, Chad

AU - Crowe, John H.

AU - Mouritsen, Ole G.

AU - Jørgensen, Kent

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Temperature-controlled atomic force microscopy (AFM) has been used to visualize and study the structure and kinetics of ripple phases in one-component dipalmitoylphosphatidylcholine (DPPC) and two-component dimyristoylphosphatidylcholine-distearoylphosphatidylcholine (DMPC-DSPC) lipid bilayers. The lipid bilayers are mica-supported double bilayers in which ripple-phase formation occurs in the top bilayer. In one-component DPPC lipid bilayers, the stable and metastable ripple phases were observed. In addition, a third ripple structure with approximately twice the wavelength of the metastable ripples was seen. From height profiles of the AFM images, estimates of the amplitudes of the different ripple phases are reported. To elucidate the processes of ripple formation and disappearance, a ripple-phase DPPC lipid bilayer was taken through the pretransition in the cooling and the heating direction and the disappearance and formation of ripples was visualized. It was found that both the disappearance and formation of ripples take place virtually one ripple at a time, thereby demonstrating the highly anisotropic nature of the ripple phase. Furthermore, when a two-component DMPC-DSPC mixture was heated from the ripple phase and into the ripple-phase/fluid-phase coexistence temperature region, the AFM images revealed that several dynamic properties of the ripple phase are important for the melting behavior of the lipid mixture. Onset of melting is observed at grain boundaries between different ripple types and different ripple orientations, and the longer-wavelength metastable ripple phase melts before the shorter-wavelength stable ripple phase. Moreover, it was observed that the ripple phase favors domain growth along the ripple direction and is responsible for creating straight-edged domains with 60° and 120° angles, as reported previously.

AB - Temperature-controlled atomic force microscopy (AFM) has been used to visualize and study the structure and kinetics of ripple phases in one-component dipalmitoylphosphatidylcholine (DPPC) and two-component dimyristoylphosphatidylcholine-distearoylphosphatidylcholine (DMPC-DSPC) lipid bilayers. The lipid bilayers are mica-supported double bilayers in which ripple-phase formation occurs in the top bilayer. In one-component DPPC lipid bilayers, the stable and metastable ripple phases were observed. In addition, a third ripple structure with approximately twice the wavelength of the metastable ripples was seen. From height profiles of the AFM images, estimates of the amplitudes of the different ripple phases are reported. To elucidate the processes of ripple formation and disappearance, a ripple-phase DPPC lipid bilayer was taken through the pretransition in the cooling and the heating direction and the disappearance and formation of ripples was visualized. It was found that both the disappearance and formation of ripples take place virtually one ripple at a time, thereby demonstrating the highly anisotropic nature of the ripple phase. Furthermore, when a two-component DMPC-DSPC mixture was heated from the ripple phase and into the ripple-phase/fluid-phase coexistence temperature region, the AFM images revealed that several dynamic properties of the ripple phase are important for the melting behavior of the lipid mixture. Onset of melting is observed at grain boundaries between different ripple types and different ripple orientations, and the longer-wavelength metastable ripple phase melts before the shorter-wavelength stable ripple phase. Moreover, it was observed that the ripple phase favors domain growth along the ripple direction and is responsible for creating straight-edged domains with 60° and 120° angles, as reported previously.

UR - http://www.scopus.com/inward/record.url?scp=0037636128&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(03)74479-8

DO - 10.1016/S0006-3495(03)74479-8

M3 - Journal article

C2 - 12829489

AN - SCOPUS:0037636128

SN - 0523-6800

VL - 85

SP - 350

EP - 360

JO - Biophysical Society. Annual Meeting. Abstracts

JF - Biophysical Society. Annual Meeting. Abstracts

IS - 1

ER -

Temperature-controlled structure and kinetics of ripple phases in one- and two-component supported lipid bilayers

Abstract

Adgang til dokumentet

Andre filer og links

Fingeraftryk

Citationsformater