Technical advance: immunophenotypical characterization of human neutrophil differentiation

Helena Isabel Mora-Jensen; Johan Jendholm; Anna Fossum; Bo T Porse; Niels Borregaard; Kim Theilgaard-Mönch

doi:10.1189/jlb.0311123

Technical advance: immunophenotypical characterization of human neutrophil differentiation

Helena Isabel Mora-Jensen, Johan Jendholm, Anna Fossum, Bo T Porse, Niels Borregaard, Kim Theilgaard-Mönch

25 Citationer (Scopus)

Abstract

The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

Originalsprog	Engelsk
Tidsskrift	Journal of Leukocyte Biology
Vol/bind	90
Udgave nummer	3
Sider (fra-til)	629-34
Antal sider	6
ISSN	0741-5400
DOI	https://doi.org/10.1189/jlb.0311123
Status	Udgivet - sep. 2011

Adgang til dokumentet

10.1189/jlb.0311123

Citationsformater

@article{7654ededeef9436098acda62435d805d,

title = "Technical advance: immunophenotypical characterization of human neutrophil differentiation",

abstract = "The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.",

author = "Mora-Jensen, {Helena Isabel} and Johan Jendholm and Anna Fossum and Porse, {Bo T} and Niels Borregaard and Kim Theilgaard-M{\"o}nch",

year = "2011",

month = sep,

doi = "10.1189/jlb.0311123",

language = "English",

volume = "90",

pages = "629--34",

journal = "Journal of Leukocyte Biology",

issn = "0741-5400",

publisher = "Federation of American Societies for Experimental Biology",

number = "3",

}

TY - JOUR

T1 - Technical advance: immunophenotypical characterization of human neutrophil differentiation

AU - Mora-Jensen, Helena Isabel

AU - Jendholm, Johan

AU - Fossum, Anna

AU - Porse, Bo T

AU - Borregaard, Niels

AU - Theilgaard-Mönch, Kim

PY - 2011/9

Y1 - 2011/9

N2 - The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

AB - The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

U2 - 10.1189/jlb.0311123

DO - 10.1189/jlb.0311123

M3 - Journal article

SN - 0741-5400

VL - 90

SP - 629

EP - 634

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

IS - 3

ER -

Technical advance: immunophenotypical characterization of human neutrophil differentiation

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater