Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing

TY - JOUR

T1 - Targeted 3' processing of antisense transcripts triggers Arabidopsis FLC chromatin silencing

AU - Liu, Fuquan

AU - Marquardt, Sebastian

AU - Lister, Clare

AU - Swiezewski, Szymon

AU - Dean, Caroline

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Noncoding RNA is emerging as an important regulator of gene expression in many organisms. We are characterizing RNA-mediated chromatin silencing of the Arabidopsis major floral repressor gene, FLC. Through suppressor mutagenesis, we identify a requirement for CstF64 and CstF77, two conserved RNA 3'-end-processing factors, in FLC silencing. However, FLC sense transcript 3' processing is not affected in the mutants. Instead, CstF64 and CstF77 are required for 3' processing of FLC antisense transcripts. A specific RNA-binding protein directs their activity to a proximal antisense polyadenylation site. This targeted processing triggers localized histone demethylase activity and results in reduced FLC sense transcription. Targeted 3' processing of antisense transcripts may be a common mechanism triggering transcriptional silencing of the corresponding sense gene.

AB - Noncoding RNA is emerging as an important regulator of gene expression in many organisms. We are characterizing RNA-mediated chromatin silencing of the Arabidopsis major floral repressor gene, FLC. Through suppressor mutagenesis, we identify a requirement for CstF64 and CstF77, two conserved RNA 3'-end-processing factors, in FLC silencing. However, FLC sense transcript 3' processing is not affected in the mutants. Instead, CstF64 and CstF77 are required for 3' processing of FLC antisense transcripts. A specific RNA-binding protein directs their activity to a proximal antisense polyadenylation site. This targeted processing triggers localized histone demethylase activity and results in reduced FLC sense transcription. Targeted 3' processing of antisense transcripts may be a common mechanism triggering transcriptional silencing of the corresponding sense gene.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Chromatin

KW - Cleavage Stimulation Factor

KW - Epistasis, Genetic

KW - Flowers

KW - Gene Expression Regulation, Plant

KW - Gene Silencing

KW - Histone Deacetylases

KW - MADS Domain Proteins

KW - Models, Genetic

KW - Polyadenylation

KW - RNA Processing, Post-Transcriptional

KW - RNA, Antisense

KW - RNA, Plant

KW - RNA-Binding Proteins

KW - Suppression, Genetic

KW - Transcription, Genetic

KW - mRNA Cleavage and Polyadenylation Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1126/science.1180278

DO - 10.1126/science.1180278

M3 - Journal article

C2 - 19965720

SN - 0036-8075

VL - 327

SP - 94

EP - 97

JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

IS - 5961

ER -