T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens

TY - JOUR

T1 - T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens

AU - Dissing, S

AU - Geisler, C

AU - Rubin, B

AU - Plesner, T

AU - Claesson, M H

N1 - Keywords: Antigens, CD3; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Calcium; Histocompatibility Antigens Class I; Humans; Interleukin-2; Lymphocyte Activation; Receptors, Antigen, T-Cell; Receptors, Interleukin-2; Signal Transduction; T-Lymphocytes

PY - 1990

Y1 - 1990

N2 - The present work demonstrates that antibody-induced cross-linking of MHC class I antigens on Jurkat T lymphoma cells leads to a rise in intracellular calcium (Cai2+) and, in the presence of phorbol ester (PMA), to IL-2 production and IL-2 receptor expression. The rise in Cai2+ exhibited a profile very different from that obtained after anti-CD3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-CD3 antibody and class I cross-linking, respectively. However, when Cai2+ was examined in individual Jurkat cells by means of a digital image processing system no differences were observed after cross-linking with anti-CD3 and anti-MHC class I antibodies, respectively. Two CD3-negative mutant lymphoma lines were nearly totally refractory to class I cross-linking. Taken together our results may indicate the existence of a functional linkage between the T cell receptor complex and MHC class I molecules.

AB - The present work demonstrates that antibody-induced cross-linking of MHC class I antigens on Jurkat T lymphoma cells leads to a rise in intracellular calcium (Cai2+) and, in the presence of phorbol ester (PMA), to IL-2 production and IL-2 receptor expression. The rise in Cai2+ exhibited a profile very different from that obtained after anti-CD3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-CD3 antibody and class I cross-linking, respectively. However, when Cai2+ was examined in individual Jurkat cells by means of a digital image processing system no differences were observed after cross-linking with anti-CD3 and anti-MHC class I antibodies, respectively. Two CD3-negative mutant lymphoma lines were nearly totally refractory to class I cross-linking. Taken together our results may indicate the existence of a functional linkage between the T cell receptor complex and MHC class I molecules.

M3 - Journal article

C2 - 2137376

SN - 0008-8749

VL - 126

SP - 196

EP - 210

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -