Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis: Optimization by response surface methodology

TY - JOUR

T1 - Synthesis of structured triacylglycerols containing caproic acid by lipase-catalyzed acidolysis

T2 - Optimization by response surface methodology

AU - Zhou, D.

AU - Xu, X.

AU - Mu, Huiling

AU - Høy, Carl-Erik

AU - Adler-Nissen, Jens

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Production in a batch reactor with a solvent-free system of structured triacylglycerols containing short-chain fatty acids by Lipozyme RM IM-catalyzed acidolysis between rapeseed oil and caproic acid was optimized using response surface methodology (RSM). Reaction time (t ), substrate ratio (S ), enzyme load (E , based on substrate), water content (W , based on enzyme), and reaction temperature (T ), the five most important parameters for the reaction, were chosen for the optimization. The range of each parameter was selected as follows: t = 5-17 h; E = 6-14 wt %; T = 45-65°C; S = 2-6 mol/mol; and W = 2-12 wt %. The biocatalyst was Lipozyme RM IM, in which Rhizomucor miehei lipase is immobilized on a resin. The incorporation of caproic acid into rapeseed oil was the main monitoring response. In addition, the contents of mono-incorporated structured triacylglycerols and di-incorporated structured triacylglycerols were also evaluated. The optimal reaction conditions for the incorporation of caproic acid and the content of di-incorporated structured triacylglycerols were as follows: t = 17 h; S = 5; E = 14 wt %; W = 10 wt %; T = 65°C. At these conditions, products with 55 mol % incorporation of caproic acid and 55 mol % di-incorporated structured triacylglycerols were obtained.

AB - Production in a batch reactor with a solvent-free system of structured triacylglycerols containing short-chain fatty acids by Lipozyme RM IM-catalyzed acidolysis between rapeseed oil and caproic acid was optimized using response surface methodology (RSM). Reaction time (t ), substrate ratio (S ), enzyme load (E , based on substrate), water content (W , based on enzyme), and reaction temperature (T ), the five most important parameters for the reaction, were chosen for the optimization. The range of each parameter was selected as follows: t = 5-17 h; E = 6-14 wt %; T = 45-65°C; S = 2-6 mol/mol; and W = 2-12 wt %. The biocatalyst was Lipozyme RM IM, in which Rhizomucor miehei lipase is immobilized on a resin. The incorporation of caproic acid into rapeseed oil was the main monitoring response. In addition, the contents of mono-incorporated structured triacylglycerols and di-incorporated structured triacylglycerols were also evaluated. The optimal reaction conditions for the incorporation of caproic acid and the content of di-incorporated structured triacylglycerols were as follows: t = 17 h; S = 5; E = 14 wt %; W = 10 wt %; T = 65°C. At these conditions, products with 55 mol % incorporation of caproic acid and 55 mol % di-incorporated structured triacylglycerols were obtained.

UR - http://www.scopus.com/inward/record.url?scp=0035543013&partnerID=8YFLogxK

U2 - 10.1021/jf0103020

DO - 10.1021/jf0103020

M3 - Journal article

AN - SCOPUS:0035543013

SN - 0021-8561

VL - 49

SP - 5771

EP - 5777

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 12

ER -