Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates

TY - JOUR

T1 - Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates

AU - Hellman, Maria

AU - Berg, Jeanette

AU - Brandt, Kristian Koefoed

AU - Hallin, Sara

PY - 2011/9

Y1 - 2011/9

N2 - Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU.

AB - Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU.

U2 - 10.1016/j.mimet.2011.05.020

DO - 10.1016/j.mimet.2011.05.020

M3 - Journal article

C2 - 21663771

SN - 0167-7012

VL - 86

SP - 376

EP - 378

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -