Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients

Rosalba Carrozzo; Daniela Verrigni; Magnhild Rasmussen; Rene de Coo; Hernan Amartino; Marzia Bianchi; Daniela Buhas; Samir Mesli; Karin Naess; Alfred Peter Born; Berit Woldseth; Paolo Prontera; Mustafa Batbayli; Kirstine Ravn; Fróði Joensen; Duccio M Cordelli; Filippo Maria Santorelli; Mar Tulinius; Niklas Darin; Morten Duno; Philippe Jouvencel; Alberto Burlina; Gabriela Stangoni; Enrico Bertini; Isabelle Redonnet-Vernhet; Flemming Wibrand; Carlo Dionisi-Vici; Johanna Uusimaa; Paivi Vieira; Andrés Nascimento Osorio; Robert McFarland; Robert W Taylor; Elisabeth Holme; Elsebet Ostergaard

doi:10.1007/s10545-015-9894-9

Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients

Rosalba Carrozzo, Daniela Verrigni, Magnhild Rasmussen, Rene de Coo, Hernan Amartino, Marzia Bianchi, Daniela Buhas, Samir Mesli, Karin Naess, Alfred Peter Born, Berit Woldseth, Paolo Prontera, Mustafa Batbayli, Kirstine Ravn, Fróði Joensen, Duccio M Cordelli, Filippo Maria Santorelli, Mar Tulinius, Niklas Darin, Morten DunoPhilippe Jouvencel, Alberto Burlina, Gabriela Stangoni, Enrico Bertini, Isabelle Redonnet-Vernhet, Flemming Wibrand, Carlo Dionisi-Vici, Johanna Uusimaa, Paivi Vieira, Andrés Nascimento Osorio, Robert McFarland, Robert W Taylor, Elisabeth Holme, Elsebet Ostergaard

Institut for Klinisk Medicin

38 Citationer (Scopus)

Abstract

BACKGROUND: The encephalomyopathic mtDNA depletion syndrome with methylmalonic aciduria is associated with deficiency of succinate-CoA ligase, caused by mutations in SUCLA2 or SUCLG1. We report here 25 new patients with succinate-CoA ligase deficiency, and review the clinical and molecular findings in these and 46 previously reported patients.

PATIENTS AND RESULTS: Of the 71 patients, 50 had SUCLA2 mutations and 21 had SUCLG1 mutations. In the newly-reported 20 SUCLA2 patients we found 16 different mutations, of which nine were novel: two large gene deletions, a 1 bp duplication, two 1 bp deletions, a 3 bp insertion, a nonsense mutation and two missense mutations. In the newly-reported SUCLG1 patients, five missense mutations were identified, of which two were novel. The median onset of symptoms was two months for patients with SUCLA2 mutations and at birth for SUCLG1 patients. Median survival was 20 years for SUCLA2 and 20 months for SUCLG1. Notable clinical differences between the two groups were hepatopathy, found in 38% of SUCLG1 cases but not in SUCLA2 cases, and hypertrophic cardiomyopathy which was not reported in SUCLA2 patients, but documented in 14% of cases with SUCLG1 mutations. Long survival, to age 20 years or older, was reported in 12% of SUCLA2 and in 10% of SUCLG1 patients. The most frequent abnormality on neuroimaging was basal ganglia involvement, found in 69% of SUCLA2 and 80% of SUCLG1 patients. Analysis of respiratory chain enzyme activities in muscle generally showed a combined deficiency of complexes I and IV, but normal histological and biochemical findings in muscle did not preclude a diagnosis of succinate-CoA ligase deficiency. In five patients, the urinary excretion of methylmalonic acid was only marginally elevated, whereas elevated plasma methylmalonic acid was consistently found.

CONCLUSIONS: To our knowledge, this is the largest study of patients with SUCLA2 and SUCLG1 deficiency. The most important findings were a significantly longer survival in patients with SUCLA2 mutations compared to SUCLG1 mutations and a trend towards longer survival in patients with missense mutations compared to loss-of-function mutations. Hypertrophic cardiomyopathy and liver involvement was exclusively found in patients with SUCLG1 mutations, whereas epilepsy was much more frequent in patients with SUCLA2 mutations compared to patients with SUCLG1 mutations. The mutation analysis revealed a number of novel mutations, including a homozygous deletion of the entire SUCLA2 gene, and we found evidence of two founder mutations in the Scandinavian population, in addition to the known SUCLA2 founder mutation in the Faroe Islands.

Originalsprog	Engelsk
Tidsskrift	Journal of Inherited Metabolic Disease
Vol/bind	39
Udgave nummer	2
Sider (fra-til)	243-52
Antal sider	10
ISSN	0141-8955
DOI	https://doi.org/10.1007/s10545-015-9894-9
Status	Udgivet - 1 mar. 2016

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10.1007/s10545-015-9894-9

Citationsformater

Carrozzo, R., Verrigni, D., Rasmussen, M., de Coo, R., Amartino, H., Bianchi, M., Buhas, D., Mesli, S., Naess, K., Born, A. P., Woldseth, B., Prontera, P., Batbayli, M., Ravn, K., Joensen, F., Cordelli, D. M., Santorelli, F. M., Tulinius, M., Darin, N., ... Ostergaard, E. (2016). Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients. Journal of Inherited Metabolic Disease, 39(2), 243-52. https://doi.org/10.1007/s10545-015-9894-9

Carrozzo, R, Verrigni, D, Rasmussen, M, de Coo, R, Amartino, H, Bianchi, M, Buhas, D, Mesli, S, Naess, K, Born, AP, Woldseth, B, Prontera, P, Batbayli, M, Ravn, K, Joensen, F, Cordelli, DM, Santorelli, FM, Tulinius, M, Darin, N, Duno, M, Jouvencel, P, Burlina, A, Stangoni, G, Bertini, E, Redonnet-Vernhet, I, Wibrand, F, Dionisi-Vici, C, Uusimaa, J, Vieira, P, Osorio, AN, McFarland, R, Taylor, RW, Holme, E & Ostergaard, E 2016, 'Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients', Journal of Inherited Metabolic Disease, bind 39, nr. 2, s. 243-52. https://doi.org/10.1007/s10545-015-9894-9

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title = "Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients",

abstract = "BACKGROUND: The encephalomyopathic mtDNA depletion syndrome with methylmalonic aciduria is associated with deficiency of succinate-CoA ligase, caused by mutations in SUCLA2 or SUCLG1. We report here 25 new patients with succinate-CoA ligase deficiency, and review the clinical and molecular findings in these and 46 previously reported patients.PATIENTS AND RESULTS: Of the 71 patients, 50 had SUCLA2 mutations and 21 had SUCLG1 mutations. In the newly-reported 20 SUCLA2 patients we found 16 different mutations, of which nine were novel: two large gene deletions, a 1 bp duplication, two 1 bp deletions, a 3 bp insertion, a nonsense mutation and two missense mutations. In the newly-reported SUCLG1 patients, five missense mutations were identified, of which two were novel. The median onset of symptoms was two months for patients with SUCLA2 mutations and at birth for SUCLG1 patients. Median survival was 20 years for SUCLA2 and 20 months for SUCLG1. Notable clinical differences between the two groups were hepatopathy, found in 38% of SUCLG1 cases but not in SUCLA2 cases, and hypertrophic cardiomyopathy which was not reported in SUCLA2 patients, but documented in 14% of cases with SUCLG1 mutations. Long survival, to age 20 years or older, was reported in 12% of SUCLA2 and in 10% of SUCLG1 patients. The most frequent abnormality on neuroimaging was basal ganglia involvement, found in 69% of SUCLA2 and 80% of SUCLG1 patients. Analysis of respiratory chain enzyme activities in muscle generally showed a combined deficiency of complexes I and IV, but normal histological and biochemical findings in muscle did not preclude a diagnosis of succinate-CoA ligase deficiency. In five patients, the urinary excretion of methylmalonic acid was only marginally elevated, whereas elevated plasma methylmalonic acid was consistently found.CONCLUSIONS: To our knowledge, this is the largest study of patients with SUCLA2 and SUCLG1 deficiency. The most important findings were a significantly longer survival in patients with SUCLA2 mutations compared to SUCLG1 mutations and a trend towards longer survival in patients with missense mutations compared to loss-of-function mutations. Hypertrophic cardiomyopathy and liver involvement was exclusively found in patients with SUCLG1 mutations, whereas epilepsy was much more frequent in patients with SUCLA2 mutations compared to patients with SUCLG1 mutations. The mutation analysis revealed a number of novel mutations, including a homozygous deletion of the entire SUCLA2 gene, and we found evidence of two founder mutations in the Scandinavian population, in addition to the known SUCLA2 founder mutation in the Faroe Islands.",

keywords = "Adolescent, Adult, Amino Acid Metabolism, Inborn Errors, Amino Acid Sequence, Child, Child, Preschool, Codon, Nonsense, DNA Mutational Analysis, DNA, Mitochondrial, Female, Genotype, Humans, Infant, Infant, Newborn, Male, Methylmalonic Acid, Mitochondrial Diseases, Mitochondrial Encephalomyopathies, Mutation, Missense, Phenotype, Succinate-CoA Ligases, Young Adult, Journal Article, Research Support, Non-U.S. Gov't",

author = "Rosalba Carrozzo and Daniela Verrigni and Magnhild Rasmussen and {de Coo}, Rene and Hernan Amartino and Marzia Bianchi and Daniela Buhas and Samir Mesli and Karin Naess and Born, {Alfred Peter} and Berit Woldseth and Paolo Prontera and Mustafa Batbayli and Kirstine Ravn and Fr{\'o}{\dh}i Joensen and Cordelli, {Duccio M} and Santorelli, {Filippo Maria} and Mar Tulinius and Niklas Darin and Morten Duno and Philippe Jouvencel and Alberto Burlina and Gabriela Stangoni and Enrico Bertini and Isabelle Redonnet-Vernhet and Flemming Wibrand and Carlo Dionisi-Vici and Johanna Uusimaa and Paivi Vieira and Osorio, {Andr{\'e}s Nascimento} and Robert McFarland and Taylor, {Robert W} and Elisabeth Holme and Elsebet Ostergaard",

year = "2016",

month = mar,

day = "1",

doi = "10.1007/s10545-015-9894-9",

language = "English",

volume = "39",

pages = "243--52",

journal = "Journal of Inherited Metabolic Disease",

issn = "0141-8955",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1

T2 - phenotype and genotype correlations in 71 patients

AU - Carrozzo, Rosalba

AU - Verrigni, Daniela

AU - Rasmussen, Magnhild

AU - de Coo, Rene

AU - Amartino, Hernan

AU - Bianchi, Marzia

AU - Buhas, Daniela

AU - Mesli, Samir

AU - Naess, Karin

AU - Born, Alfred Peter

AU - Woldseth, Berit

AU - Prontera, Paolo

AU - Batbayli, Mustafa

AU - Ravn, Kirstine

AU - Joensen, Fróði

AU - Cordelli, Duccio M

AU - Santorelli, Filippo Maria

AU - Tulinius, Mar

AU - Darin, Niklas

AU - Duno, Morten

AU - Jouvencel, Philippe

AU - Burlina, Alberto

AU - Stangoni, Gabriela

AU - Bertini, Enrico

AU - Redonnet-Vernhet, Isabelle

AU - Wibrand, Flemming

AU - Dionisi-Vici, Carlo

AU - Uusimaa, Johanna

AU - Vieira, Paivi

AU - Osorio, Andrés Nascimento

AU - McFarland, Robert

AU - Taylor, Robert W

AU - Holme, Elisabeth

AU - Ostergaard, Elsebet

PY - 2016/3/1

Y1 - 2016/3/1

N2 - BACKGROUND: The encephalomyopathic mtDNA depletion syndrome with methylmalonic aciduria is associated with deficiency of succinate-CoA ligase, caused by mutations in SUCLA2 or SUCLG1. We report here 25 new patients with succinate-CoA ligase deficiency, and review the clinical and molecular findings in these and 46 previously reported patients.PATIENTS AND RESULTS: Of the 71 patients, 50 had SUCLA2 mutations and 21 had SUCLG1 mutations. In the newly-reported 20 SUCLA2 patients we found 16 different mutations, of which nine were novel: two large gene deletions, a 1 bp duplication, two 1 bp deletions, a 3 bp insertion, a nonsense mutation and two missense mutations. In the newly-reported SUCLG1 patients, five missense mutations were identified, of which two were novel. The median onset of symptoms was two months for patients with SUCLA2 mutations and at birth for SUCLG1 patients. Median survival was 20 years for SUCLA2 and 20 months for SUCLG1. Notable clinical differences between the two groups were hepatopathy, found in 38% of SUCLG1 cases but not in SUCLA2 cases, and hypertrophic cardiomyopathy which was not reported in SUCLA2 patients, but documented in 14% of cases with SUCLG1 mutations. Long survival, to age 20 years or older, was reported in 12% of SUCLA2 and in 10% of SUCLG1 patients. The most frequent abnormality on neuroimaging was basal ganglia involvement, found in 69% of SUCLA2 and 80% of SUCLG1 patients. Analysis of respiratory chain enzyme activities in muscle generally showed a combined deficiency of complexes I and IV, but normal histological and biochemical findings in muscle did not preclude a diagnosis of succinate-CoA ligase deficiency. In five patients, the urinary excretion of methylmalonic acid was only marginally elevated, whereas elevated plasma methylmalonic acid was consistently found.CONCLUSIONS: To our knowledge, this is the largest study of patients with SUCLA2 and SUCLG1 deficiency. The most important findings were a significantly longer survival in patients with SUCLA2 mutations compared to SUCLG1 mutations and a trend towards longer survival in patients with missense mutations compared to loss-of-function mutations. Hypertrophic cardiomyopathy and liver involvement was exclusively found in patients with SUCLG1 mutations, whereas epilepsy was much more frequent in patients with SUCLA2 mutations compared to patients with SUCLG1 mutations. The mutation analysis revealed a number of novel mutations, including a homozygous deletion of the entire SUCLA2 gene, and we found evidence of two founder mutations in the Scandinavian population, in addition to the known SUCLA2 founder mutation in the Faroe Islands.

AB - BACKGROUND: The encephalomyopathic mtDNA depletion syndrome with methylmalonic aciduria is associated with deficiency of succinate-CoA ligase, caused by mutations in SUCLA2 or SUCLG1. We report here 25 new patients with succinate-CoA ligase deficiency, and review the clinical and molecular findings in these and 46 previously reported patients.PATIENTS AND RESULTS: Of the 71 patients, 50 had SUCLA2 mutations and 21 had SUCLG1 mutations. In the newly-reported 20 SUCLA2 patients we found 16 different mutations, of which nine were novel: two large gene deletions, a 1 bp duplication, two 1 bp deletions, a 3 bp insertion, a nonsense mutation and two missense mutations. In the newly-reported SUCLG1 patients, five missense mutations were identified, of which two were novel. The median onset of symptoms was two months for patients with SUCLA2 mutations and at birth for SUCLG1 patients. Median survival was 20 years for SUCLA2 and 20 months for SUCLG1. Notable clinical differences between the two groups were hepatopathy, found in 38% of SUCLG1 cases but not in SUCLA2 cases, and hypertrophic cardiomyopathy which was not reported in SUCLA2 patients, but documented in 14% of cases with SUCLG1 mutations. Long survival, to age 20 years or older, was reported in 12% of SUCLA2 and in 10% of SUCLG1 patients. The most frequent abnormality on neuroimaging was basal ganglia involvement, found in 69% of SUCLA2 and 80% of SUCLG1 patients. Analysis of respiratory chain enzyme activities in muscle generally showed a combined deficiency of complexes I and IV, but normal histological and biochemical findings in muscle did not preclude a diagnosis of succinate-CoA ligase deficiency. In five patients, the urinary excretion of methylmalonic acid was only marginally elevated, whereas elevated plasma methylmalonic acid was consistently found.CONCLUSIONS: To our knowledge, this is the largest study of patients with SUCLA2 and SUCLG1 deficiency. The most important findings were a significantly longer survival in patients with SUCLA2 mutations compared to SUCLG1 mutations and a trend towards longer survival in patients with missense mutations compared to loss-of-function mutations. Hypertrophic cardiomyopathy and liver involvement was exclusively found in patients with SUCLG1 mutations, whereas epilepsy was much more frequent in patients with SUCLA2 mutations compared to patients with SUCLG1 mutations. The mutation analysis revealed a number of novel mutations, including a homozygous deletion of the entire SUCLA2 gene, and we found evidence of two founder mutations in the Scandinavian population, in addition to the known SUCLA2 founder mutation in the Faroe Islands.

KW - Adolescent

KW - Adult

KW - Amino Acid Metabolism, Inborn Errors

KW - Amino Acid Sequence

KW - Child

KW - Child, Preschool

KW - Codon, Nonsense

KW - DNA Mutational Analysis

KW - DNA, Mitochondrial

KW - Female

KW - Genotype

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Male

KW - Methylmalonic Acid

KW - Mitochondrial Diseases

KW - Mitochondrial Encephalomyopathies

KW - Mutation, Missense

KW - Phenotype

KW - Succinate-CoA Ligases

KW - Young Adult

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s10545-015-9894-9

DO - 10.1007/s10545-015-9894-9

M3 - Journal article

C2 - 26475597

SN - 0141-8955

VL - 39

SP - 243

EP - 252

JO - Journal of Inherited Metabolic Disease

JF - Journal of Inherited Metabolic Disease

IS - 2

ER -

Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients

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