Substoichiometric ribose methylations in spliceosomal snRNAs

TY - JOUR

T1 - Substoichiometric ribose methylations in spliceosomal snRNAs

AU - Krogh, Nicolai

AU - Kongsbak-Wismann, Martin

AU - Geisler, Carsten

AU - Nielsen, Henrik

PY - 2017

Y1 - 2017

N2 - Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.

AB - Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.

U2 - 10.1039/c7ob02317k

DO - 10.1039/c7ob02317k

M3 - Journal article

C2 - 29048444

AN - SCOPUS:85032730824

SN - 1477-0520

VL - 15

SP - 8872

EP - 8876

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 42

ER -