Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms

Klaus Harms; Asbjørn Lunnan; Nils Hülter; Tobias Mourier; Lasse Vinner; Cheryl P. Andam; Pekka Marttinen; Eva Marie Helena Fridholm; Anders Johannes Hansen; William P. Hanage; Kaare Magne Nielsen; Eske Willerslev; Pål Jarle Johnsen

doi:10.1073/pnas.1615819114

Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms

Klaus Harms, Asbjørn Lunnan, Nils Hülter, Tobias Mourier, Lasse Vinner, Cheryl P. Andam, Pekka Marttinen, Eva Marie Helena Fridholm, Anders Johannes Hansen, William P. Hanage, Kaare Magne Nielsen, Eske Willerslev, Pål Jarle Johnsen

5 Citationer (Scopus)

Abstract

In a screen for unexplained mutation events we identified a previously unrecognized mechanism generating clustered DNA polymorphisms such as microindels and cumulative SNPs. The mechanism, short-patch double illegitimate recombination (SPDIR), facilitates short single-stranded DNA molecules to invade and replace genomic DNA through two joint illegitimate recombination events. SPDIR is controlled by key components of the cellular genome maintenance machinery in the gram-negative bacterium Acinetobacter baylyi. The source DNA is primarily intragenomic but can also be acquired through horizontal gene transfer. The DNA replacements are nonreciprocal and locus independent. Bioinformatic approaches reveal occurrence of SPDIR events in the gram-positive human pathogen Streptococcus pneumoniae and in the human genome.

Originalsprog	Engelsk
Tidsskrift	National Academy of Sciences. Proceedings
Vol/bind	113
Udgave nummer	52
Sider (fra-til)	15066-15071
Antal sider	6
ISSN	0027-8424
DOI	https://doi.org/10.1073/pnas.1615819114
Status	Udgivet - 27 dec. 2016

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10.1073/pnas.1615819114

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Harms, K., Lunnan, A., Hülter, N., Mourier, T., Vinner, L., Andam, C. P., Marttinen, P., Fridholm, E. M. H., Hansen, A. J., Hanage, W. P., Nielsen, K. M., Willerslev, E., & Johnsen, P. J. (2016). Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms. National Academy of Sciences. Proceedings, 113(52), 15066-15071. https://doi.org/10.1073/pnas.1615819114

Harms, K, Lunnan, A, Hülter, N, Mourier, T, Vinner, L, Andam, CP, Marttinen, P, Fridholm, EMH, Hansen, AJ, Hanage, WP, Nielsen, KM, Willerslev, E & Johnsen, PJ 2016, 'Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms', National Academy of Sciences. Proceedings, bind 113, nr. 52, s. 15066-15071. https://doi.org/10.1073/pnas.1615819114

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title = "Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms",

abstract = "In a screen for unexplained mutation events we identified a previously unrecognized mechanism generating clustered DNA polymorphisms such as microindels and cumulative SNPs. The mechanism, short-patch double illegitimate recombination (SPDIR), facilitates short single-stranded DNA molecules to invade and replace genomic DNA through two joint illegitimate recombination events. SPDIR is controlled by key components of the cellular genome maintenance machinery in the gram-negative bacterium Acinetobacter baylyi. The source DNA is primarily intragenomic but can also be acquired through horizontal gene transfer. The DNA replacements are nonreciprocal and locus independent. Bioinformatic approaches reveal occurrence of SPDIR events in the gram-positive human pathogen Streptococcus pneumoniae and in the human genome.",

author = "Klaus Harms and Asbj{\o}rn Lunnan and Nils H{\"u}lter and Tobias Mourier and Lasse Vinner and Andam, {Cheryl P.} and Pekka Marttinen and Fridholm, {Eva Marie Helena} and Hansen, {Anders Johannes} and Hanage, {William P.} and Nielsen, {Kaare Magne} and Eske Willerslev and Johnsen, {P{\aa}l Jarle}",

year = "2016",

month = dec,

day = "27",

doi = "10.1073/pnas.1615819114",

language = "English",

volume = "113",

pages = "15066--15071",

journal = "National Academy of Sciences. Proceedings",

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T1 - Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms

AU - Harms, Klaus

AU - Lunnan, Asbjørn

AU - Hülter, Nils

AU - Mourier, Tobias

AU - Vinner, Lasse

AU - Andam, Cheryl P.

AU - Marttinen, Pekka

AU - Fridholm, Eva Marie Helena

AU - Hansen, Anders Johannes

AU - Hanage, William P.

AU - Nielsen, Kaare Magne

AU - Willerslev, Eske

AU - Johnsen, Pål Jarle

PY - 2016/12/27

Y1 - 2016/12/27

N2 - In a screen for unexplained mutation events we identified a previously unrecognized mechanism generating clustered DNA polymorphisms such as microindels and cumulative SNPs. The mechanism, short-patch double illegitimate recombination (SPDIR), facilitates short single-stranded DNA molecules to invade and replace genomic DNA through two joint illegitimate recombination events. SPDIR is controlled by key components of the cellular genome maintenance machinery in the gram-negative bacterium Acinetobacter baylyi. The source DNA is primarily intragenomic but can also be acquired through horizontal gene transfer. The DNA replacements are nonreciprocal and locus independent. Bioinformatic approaches reveal occurrence of SPDIR events in the gram-positive human pathogen Streptococcus pneumoniae and in the human genome.

AB - In a screen for unexplained mutation events we identified a previously unrecognized mechanism generating clustered DNA polymorphisms such as microindels and cumulative SNPs. The mechanism, short-patch double illegitimate recombination (SPDIR), facilitates short single-stranded DNA molecules to invade and replace genomic DNA through two joint illegitimate recombination events. SPDIR is controlled by key components of the cellular genome maintenance machinery in the gram-negative bacterium Acinetobacter baylyi. The source DNA is primarily intragenomic but can also be acquired through horizontal gene transfer. The DNA replacements are nonreciprocal and locus independent. Bioinformatic approaches reveal occurrence of SPDIR events in the gram-positive human pathogen Streptococcus pneumoniae and in the human genome.

U2 - 10.1073/pnas.1615819114

DO - 10.1073/pnas.1615819114

M3 - Journal article

C2 - 27956618

SN - 0027-8424

VL - 113

SP - 15066

EP - 15071

JO - National Academy of Sciences. Proceedings

JF - National Academy of Sciences. Proceedings

IS - 52

ER -

Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms

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