Studies on the native forms of renin in the rat kidney

TY - JOUR

T1 - Studies on the native forms of renin in the rat kidney

AU - Rubin, Inger

AU - Lauritzen, Edgar

AU - Lauritzen, Martin

PY - 1980/3/14

Y1 - 1980/3/14

N2 - This paper describes Sephadex G-100 chromatography of rat kidney extract containing various enzyme inhibitors. The high molecular weight renin (molecular weight above 50 000) constitutes about 50% of the total renin activity. Omission of the enzyme inhibitors yield solely low molecular weight renin. Upon rechromatography high molecular weight renin eluted in two peaks at lower molecular weight with a concomitant reduction of renin activity. Renin activity in the fractions from Sephadex G-100 chromatography was increased 70% by dialysis at acid as well as neutral pH through the whole molecular weight range. Cold storage of extract with low molecular weight increased renin activity about 25%. The results suggest that the fully active enzyme is not represented by the lower molecular weight forms of renin and direct connection between activation of renin and reduction of renin molecular size was not indicated.

AB - This paper describes Sephadex G-100 chromatography of rat kidney extract containing various enzyme inhibitors. The high molecular weight renin (molecular weight above 50 000) constitutes about 50% of the total renin activity. Omission of the enzyme inhibitors yield solely low molecular weight renin. Upon rechromatography high molecular weight renin eluted in two peaks at lower molecular weight with a concomitant reduction of renin activity. Renin activity in the fractions from Sephadex G-100 chromatography was increased 70% by dialysis at acid as well as neutral pH through the whole molecular weight range. Cold storage of extract with low molecular weight increased renin activity about 25%. The results suggest that the fully active enzyme is not represented by the lower molecular weight forms of renin and direct connection between activation of renin and reduction of renin molecular size was not indicated.

KW - (Rat kidney)

KW - Molecular forms

KW - Renin

UR - http://www.scopus.com/inward/record.url?scp=0019152694&partnerID=8YFLogxK

U2 - 10.1016/0005-2744(80)90285-5

DO - 10.1016/0005-2744(80)90285-5

M3 - Journal article

C2 - 6988009

AN - SCOPUS:0019152694

SN - 0924-1086

VL - 612

SP - 126

EP - 136

JO - BBA - Enzymology

JF - BBA - Enzymology

IS - 1

ER -