Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant

TY - JOUR

T1 - Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant

AU - Lo Leggio, Leila

AU - Weihe, Cecilia D.

AU - Poulsen, Jens-christian N.

AU - Sweeney, Matt

AU - Rasmussen, Frank

AU - Lin, Janine

AU - De Maria, Leonardo

AU - Wogulis, Mark

PY - 2018/11

Y1 - 2018/11

N2 - Lytic polysaccharide monooxygenases (LPMOs) are industrial enzymes which are gaining use in second generation bioethanol production from lignocellulose by acting in synergy with glycoside hydrolases. Here we present the X-ray crystal structure of an AA9 fungal LPMO from Aspergillus fumigatus and a variant which has been shown to have better performance at elevated temperatures. Based on the structures, thermal denaturation data and theoretical calculations, we provide a suggestion for the structural basis of the improved stability.

AB - Lytic polysaccharide monooxygenases (LPMOs) are industrial enzymes which are gaining use in second generation bioethanol production from lignocellulose by acting in synergy with glycoside hydrolases. Here we present the X-ray crystal structure of an AA9 fungal LPMO from Aspergillus fumigatus and a variant which has been shown to have better performance at elevated temperatures. Based on the structures, thermal denaturation data and theoretical calculations, we provide a suggestion for the structural basis of the improved stability.

U2 - 10.1016/j.carres.2018.08.009

DO - 10.1016/j.carres.2018.08.009

M3 - Journal article

C2 - 30296642

SN - 0008-6215

VL - 469

SP - 55

EP - 59

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -