Structure elucidation and quantification of impurities formed between 6-aminocaproic acid and the excipients citric acid and sorbitol in an oral solution using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy

Anne Marie V Schou-Pedersen; Claus Cornett; Nils Nyberg; Jesper Østergaard; Steen Honoré Hansen

doi:10.1016/j.jpba.2015.01.022

Structure elucidation and quantification of impurities formed between 6-aminocaproic acid and the excipients citric acid and sorbitol in an oral solution using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy

Anne Marie V Schou-Pedersen, Claus Cornett, Nils Nyberg, Jesper Østergaard, Steen Honoré Hansen

5 Citationer (Scopus)

Abstract

Concentrated solutions containing 6-aminocaproic acid and the excipients citric acid and sorbitol have been studied at temperatures of 50 °C, 60 °C, 70 °C and 80 °C as well as at 20 °C. It has previously been reported that the commonly employed citric acid is a reactive excipient, and it is therefore important to thoroughly investigate a possible reaction between 6-aminocaproic acid and citric acid. The current study revealed the formation of 3-hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid between 6-aminocaproic acid and citric acid by high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance spectroscopy (NMR). Less than 0.03% of 6-aminocaproic acid was converted to 3-hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid after 30 days of storage at 80 °C. Degradation products of 6-aminocaproic acid were also observed after storage at the applied temperatures, e.g., dimer, trimer and cyclized 6-aminocaproic acid, i.e., caprolactam. No reaction products between d-sorbitol and 6-aminocaproic acid could be observed. 3-Hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid, dimer and caprolactam were also observed after storage at 20 °C for 3 months. The findings imply that an oral solution of 6-aminocaproic acid is relatively stable at 20 °C at the pH values 4.00 and 5.00 as suggested in the USP for oral formulations. Compliance with the ICH guideline Q3B is expected.

Originalsprog	Engelsk
Tidsskrift	Journal of Pharmaceutical and Biomedical Analysis
Vol/bind	107C
Sider (fra-til)	333-340
Antal sider	8
ISSN	0731-7085
DOI	https://doi.org/10.1016/j.jpba.2015.01.022
Status	Udgivet - 5 mar. 2015

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10.1016/j.jpba.2015.01.022

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Citationsformater

Schou-Pedersen, A. M. V., Cornett, C., Nyberg, N., Østergaard, J., & Hansen, S. H. (2015). Structure elucidation and quantification of impurities formed between 6-aminocaproic acid and the excipients citric acid and sorbitol in an oral solution using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. Journal of Pharmaceutical and Biomedical Analysis, 107C, 333-340. https://doi.org/10.1016/j.jpba.2015.01.022

Structure elucidation and quantification of impurities formed between 6-aminocaproic acid and the excipients citric acid and sorbitol in an oral solution using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. / Schou-Pedersen, Anne Marie V ; Cornett, Claus; Nyberg, Nils et al.
I: Journal of Pharmaceutical and Biomedical Analysis, Bind 107C, 05.03.2015, s. 333-340.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Schou-Pedersen, AMV , Cornett, C, Nyberg, N, Østergaard, J & Hansen, SH 2015, 'Structure elucidation and quantification of impurities formed between 6-aminocaproic acid and the excipients citric acid and sorbitol in an oral solution using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy', Journal of Pharmaceutical and Biomedical Analysis, bind 107C, s. 333-340. https://doi.org/10.1016/j.jpba.2015.01.022

Schou-Pedersen AMV , Cornett C, Nyberg N, Østergaard J, Hansen SH. Structure elucidation and quantification of impurities formed between 6-aminocaproic acid and the excipients citric acid and sorbitol in an oral solution using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. Journal of Pharmaceutical and Biomedical Analysis. 2015 mar. 5;107C:333-340. doi: 10.1016/j.jpba.2015.01.022

Schou-Pedersen, Anne Marie V ; Cornett, Claus ; Nyberg, Nils et al. / Structure elucidation and quantification of impurities formed between 6-aminocaproic acid and the excipients citric acid and sorbitol in an oral solution using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. I: Journal of Pharmaceutical and Biomedical Analysis. 2015 ; Bind 107C. s. 333-340.

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abstract = "Concentrated solutions containing 6-aminocaproic acid and the excipients citric acid and sorbitol have been studied at temperatures of 50 °C, 60 °C, 70 °C and 80 °C as well as at 20 °C. It has previously been reported that the commonly employed citric acid is a reactive excipient, and it is therefore important to thoroughly investigate a possible reaction between 6-aminocaproic acid and citric acid. The current study revealed the formation of 3-hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid between 6-aminocaproic acid and citric acid by high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance spectroscopy (NMR). Less than 0.03% of 6-aminocaproic acid was converted to 3-hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid after 30 days of storage at 80 °C. Degradation products of 6-aminocaproic acid were also observed after storage at the applied temperatures, e.g., dimer, trimer and cyclized 6-aminocaproic acid, i.e., caprolactam. No reaction products between d-sorbitol and 6-aminocaproic acid could be observed. 3-Hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid, dimer and caprolactam were also observed after storage at 20 °C for 3 months. The findings imply that an oral solution of 6-aminocaproic acid is relatively stable at 20 °C at the pH values 4.00 and 5.00 as suggested in the USP for oral formulations. Compliance with the ICH guideline Q3B is expected.",

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AU - Schou-Pedersen, Anne Marie V

AU - Cornett, Claus

AU - Nyberg, Nils

AU - Østergaard, Jesper

AU - Hansen, Steen Honoré

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N2 - Concentrated solutions containing 6-aminocaproic acid and the excipients citric acid and sorbitol have been studied at temperatures of 50 °C, 60 °C, 70 °C and 80 °C as well as at 20 °C. It has previously been reported that the commonly employed citric acid is a reactive excipient, and it is therefore important to thoroughly investigate a possible reaction between 6-aminocaproic acid and citric acid. The current study revealed the formation of 3-hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid between 6-aminocaproic acid and citric acid by high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance spectroscopy (NMR). Less than 0.03% of 6-aminocaproic acid was converted to 3-hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid after 30 days of storage at 80 °C. Degradation products of 6-aminocaproic acid were also observed after storage at the applied temperatures, e.g., dimer, trimer and cyclized 6-aminocaproic acid, i.e., caprolactam. No reaction products between d-sorbitol and 6-aminocaproic acid could be observed. 3-Hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid, dimer and caprolactam were also observed after storage at 20 °C for 3 months. The findings imply that an oral solution of 6-aminocaproic acid is relatively stable at 20 °C at the pH values 4.00 and 5.00 as suggested in the USP for oral formulations. Compliance with the ICH guideline Q3B is expected.

AB - Concentrated solutions containing 6-aminocaproic acid and the excipients citric acid and sorbitol have been studied at temperatures of 50 °C, 60 °C, 70 °C and 80 °C as well as at 20 °C. It has previously been reported that the commonly employed citric acid is a reactive excipient, and it is therefore important to thoroughly investigate a possible reaction between 6-aminocaproic acid and citric acid. The current study revealed the formation of 3-hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid between 6-aminocaproic acid and citric acid by high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance spectroscopy (NMR). Less than 0.03% of 6-aminocaproic acid was converted to 3-hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid after 30 days of storage at 80 °C. Degradation products of 6-aminocaproic acid were also observed after storage at the applied temperatures, e.g., dimer, trimer and cyclized 6-aminocaproic acid, i.e., caprolactam. No reaction products between d-sorbitol and 6-aminocaproic acid could be observed. 3-Hydroxy-3,4-dicarboxy-butanamide-N-hexanoic acid, dimer and caprolactam were also observed after storage at 20 °C for 3 months. The findings imply that an oral solution of 6-aminocaproic acid is relatively stable at 20 °C at the pH values 4.00 and 5.00 as suggested in the USP for oral formulations. Compliance with the ICH guideline Q3B is expected.

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DO - 10.1016/j.jpba.2015.01.022

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