Stokes shift microscopy by excitation and emission imaging

TY - JOUR

T1 - Stokes shift microscopy by excitation and emission imaging

AU - Krause, S.

AU - Vosch, T.

PY - 2019

Y1 - 2019

N2 - In this contribution, we present a new method, based on a tunable excitation laser source and a robust common path interferometer in the detection channel. Its purpose is to image spectral excitation and emission information on a monochrome complementary metal oxide semiconductor (CMOS) camera. This allows us to spatially obtain both excitation and emission spectra of the whole imaged area and create derived images such as red-green-blue (RGB), excitation and emission maxima, and Stokes shift images. Our presented method is a further development of hyperspectral imaging that usually is limited to recording spatially resolved emission spectra. Taking advantage of the full camera chip should speed up the acquisition versus line scan or pointwise hyperspectral imaging.

AB - In this contribution, we present a new method, based on a tunable excitation laser source and a robust common path interferometer in the detection channel. Its purpose is to image spectral excitation and emission information on a monochrome complementary metal oxide semiconductor (CMOS) camera. This allows us to spatially obtain both excitation and emission spectra of the whole imaged area and create derived images such as red-green-blue (RGB), excitation and emission maxima, and Stokes shift images. Our presented method is a further development of hyperspectral imaging that usually is limited to recording spatially resolved emission spectra. Taking advantage of the full camera chip should speed up the acquisition versus line scan or pointwise hyperspectral imaging.

U2 - 10.1364/OE.27.008208

DO - 10.1364/OE.27.008208

M3 - Journal article

C2 - 31052643

SN - 1094-4087

VL - 27

SP - 8208

EP - 8220

JO - Optics Express

JF - Optics Express

IS - 6

ER -