Starch granules size distribution in superior and inferior grains of wheat is related to enzyme activities and their gene expressions during grain filling

TY - JOUR

T1 - Starch granules size distribution in superior and inferior grains of wheat is related to enzyme activities and their gene expressions during grain filling

AU - Zhang, Chuanhui

AU - Jiang, Dong

AU - Liu, Fulai

AU - Cai, Jian

AU - Dai, Tingbo

AU - Cao, Weixing

PY - 2010/3

Y1 - 2010/3

N2 - Mature wheat endosperm contains A-, B-, C-type starch granules, and each class has unique physiochemicalproperties which determine the quality of starch. The dynamics of the starch granule sizedistribution, activities of starch synthases and expression of starch synthase encoding genes werestudied in superior and inferior grains during grain filling. Compared with inferior grains, superior grainsshowed higher grain weight, contents of starch, amylose and amylopectin. The formation of A-, B-, C-typestarch granules initiated at 4, 8, 20 DAF, respectively, and was well consistent with the temporally changepatterns of starch synthase activities and relative gene expression levels. For instance, activities ofsoluble and granule-bound starch synthases (designated SSS and GBSS) peaked at 20 and 24 DAF. Genesencoding isoforms of starch synthases expressed at different grain filling periods. In addition, SS I wasgenerally expressed over the grain filling stage; the SS II and SS III were expressed over the early and midgrain filling stage, and the GBSS I was expressed during the mid to late grain filling stage. In addition, thetime-course changes in activities of starch synthases and expression of starch synthase encoding genesexplained well the dynamics of the starch granule size distribution.

AB - Mature wheat endosperm contains A-, B-, C-type starch granules, and each class has unique physiochemicalproperties which determine the quality of starch. The dynamics of the starch granule sizedistribution, activities of starch synthases and expression of starch synthase encoding genes werestudied in superior and inferior grains during grain filling. Compared with inferior grains, superior grainsshowed higher grain weight, contents of starch, amylose and amylopectin. The formation of A-, B-, C-typestarch granules initiated at 4, 8, 20 DAF, respectively, and was well consistent with the temporally changepatterns of starch synthase activities and relative gene expression levels. For instance, activities ofsoluble and granule-bound starch synthases (designated SSS and GBSS) peaked at 20 and 24 DAF. Genesencoding isoforms of starch synthases expressed at different grain filling periods. In addition, SS I wasgenerally expressed over the grain filling stage; the SS II and SS III were expressed over the early and midgrain filling stage, and the GBSS I was expressed during the mid to late grain filling stage. In addition, thetime-course changes in activities of starch synthases and expression of starch synthase encoding genesexplained well the dynamics of the starch granule size distribution.

U2 - 10.1016/j.jcs.2009.12.002

DO - 10.1016/j.jcs.2009.12.002

M3 - Journal article

SN - 0733-5210

VL - 51

SP - 226

EP - 233

JO - Journal of Cereal Science

JF - Journal of Cereal Science

IS - 2

ER -