Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis

Katarzyna Koltowska; Holger Apitz; Despina Stamataki; Elizabeth M A Hirst; Heather Verkade; Iris Salecker; Elke Ober

doi:10.1242/dev.093583

Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis

Katarzyna Koltowska, Holger Apitz, Despina Stamataki, Elizabeth M A Hirst, Heather Verkade, Iris Salecker, Elke Ober

14 Citationer (Scopus)

Abstract

Tightly controlled DNA replication and RNA transcription are essential for differentiation and tissue growth in multicellular organisms. Histone chaperones, including the FACT (facilitates chromatin transcription) complex, are central for these processes and act by mediating DNA access through nucleosome reorganisation. However, their roles in vertebrate organogenesis are poorly understood. Here, we report the identification of zebrafish mutants for the gene encoding Structure specific recognition protein 1a (Ssrp1a), which, together with Spt16, forms the FACT heterodimer. Focussing on the liver and eye, we show that zygotic Ssrp1a is essential for proliferation and differentiation during organogenesis. Specifically, gene expression indicative of progressive organ differentiation is disrupted and RNA transcription is globally reduced. Ssrp1a-deficient embryos exhibit DNA synthesis defects and prolonged S phase, uncovering a role distinct from that of Spt16, which promotes G1 phase progression. Gene deletion/replacement experiments in Drosophila show that Ssrp1b, Ssrp1a and N-terminal Ssrp1a, equivalent to the yeast homologue Pob3, can substitute Drosophila Ssrp function. These data suggest that (1) Ssrp1b does not compensate for Ssrp1a loss in the zebrafish embryo, probably owing to insufficient expression levels, and (2) despite fundamental structural differences, the mechanisms mediating DNA accessibility by FACT are conserved between yeast and metazoans. We propose that the essential functions of Ssrp1a in DNA replication and gene transcription, together with its dynamic spatiotemporal expression, ensure organ-specific differentiation and proportional growth, which are crucial for the forming embryo.

Originalsprog	Engelsk
Tidsskrift	Development (Cambridge, England)
Vol/bind	140
Udgave nummer	9
Sider (fra-til)	1912-8
Antal sider	7
ISSN	0950-1991
DOI	https://doi.org/10.1242/dev.093583
Status	Udgivet - 1 maj 2013
Udgivet eksternt	Ja

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10.1242/dev.093583

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title = "Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis",

abstract = "Tightly controlled DNA replication and RNA transcription are essential for differentiation and tissue growth in multicellular organisms. Histone chaperones, including the FACT (facilitates chromatin transcription) complex, are central for these processes and act by mediating DNA access through nucleosome reorganisation. However, their roles in vertebrate organogenesis are poorly understood. Here, we report the identification of zebrafish mutants for the gene encoding Structure specific recognition protein 1a (Ssrp1a), which, together with Spt16, forms the FACT heterodimer. Focussing on the liver and eye, we show that zygotic Ssrp1a is essential for proliferation and differentiation during organogenesis. Specifically, gene expression indicative of progressive organ differentiation is disrupted and RNA transcription is globally reduced. Ssrp1a-deficient embryos exhibit DNA synthesis defects and prolonged S phase, uncovering a role distinct from that of Spt16, which promotes G1 phase progression. Gene deletion/replacement experiments in Drosophila show that Ssrp1b, Ssrp1a and N-terminal Ssrp1a, equivalent to the yeast homologue Pob3, can substitute Drosophila Ssrp function. These data suggest that (1) Ssrp1b does not compensate for Ssrp1a loss in the zebrafish embryo, probably owing to insufficient expression levels, and (2) despite fundamental structural differences, the mechanisms mediating DNA accessibility by FACT are conserved between yeast and metazoans. We propose that the essential functions of Ssrp1a in DNA replication and gene transcription, together with its dynamic spatiotemporal expression, ensure organ-specific differentiation and proportional growth, which are crucial for the forming embryo.",

keywords = "Animals, Cell Cycle, Cell Proliferation, Chromatin Assembly and Disassembly, DNA Replication, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Embryo, Nonmammalian, Endoderm, Eye, Female, Gene Expression Regulation, Developmental, High Mobility Group Proteins, Imaginal Discs, Liver, Male, Mitotic Index, Mutation, Organogenesis, RNA, Transcription, Genetic, Transcriptional Elongation Factors, Zebrafish, Zebrafish Proteins",

author = "Katarzyna Koltowska and Holger Apitz and Despina Stamataki and Hirst, {Elizabeth M A} and Heather Verkade and Iris Salecker and Elke Ober",

year = "2013",

month = may,

day = "1",

doi = "10.1242/dev.093583",

language = "English",

volume = "140",

pages = "1912--8",

journal = "Development (Cambridge, England)",

issn = "0950-1991",

publisher = "The Company of Biologists",

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TY - JOUR

T1 - Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis

AU - Koltowska, Katarzyna

AU - Apitz, Holger

AU - Stamataki, Despina

AU - Hirst, Elizabeth M A

AU - Verkade, Heather

AU - Salecker, Iris

AU - Ober, Elke

PY - 2013/5/1

Y1 - 2013/5/1

N2 - Tightly controlled DNA replication and RNA transcription are essential for differentiation and tissue growth in multicellular organisms. Histone chaperones, including the FACT (facilitates chromatin transcription) complex, are central for these processes and act by mediating DNA access through nucleosome reorganisation. However, their roles in vertebrate organogenesis are poorly understood. Here, we report the identification of zebrafish mutants for the gene encoding Structure specific recognition protein 1a (Ssrp1a), which, together with Spt16, forms the FACT heterodimer. Focussing on the liver and eye, we show that zygotic Ssrp1a is essential for proliferation and differentiation during organogenesis. Specifically, gene expression indicative of progressive organ differentiation is disrupted and RNA transcription is globally reduced. Ssrp1a-deficient embryos exhibit DNA synthesis defects and prolonged S phase, uncovering a role distinct from that of Spt16, which promotes G1 phase progression. Gene deletion/replacement experiments in Drosophila show that Ssrp1b, Ssrp1a and N-terminal Ssrp1a, equivalent to the yeast homologue Pob3, can substitute Drosophila Ssrp function. These data suggest that (1) Ssrp1b does not compensate for Ssrp1a loss in the zebrafish embryo, probably owing to insufficient expression levels, and (2) despite fundamental structural differences, the mechanisms mediating DNA accessibility by FACT are conserved between yeast and metazoans. We propose that the essential functions of Ssrp1a in DNA replication and gene transcription, together with its dynamic spatiotemporal expression, ensure organ-specific differentiation and proportional growth, which are crucial for the forming embryo.

AB - Tightly controlled DNA replication and RNA transcription are essential for differentiation and tissue growth in multicellular organisms. Histone chaperones, including the FACT (facilitates chromatin transcription) complex, are central for these processes and act by mediating DNA access through nucleosome reorganisation. However, their roles in vertebrate organogenesis are poorly understood. Here, we report the identification of zebrafish mutants for the gene encoding Structure specific recognition protein 1a (Ssrp1a), which, together with Spt16, forms the FACT heterodimer. Focussing on the liver and eye, we show that zygotic Ssrp1a is essential for proliferation and differentiation during organogenesis. Specifically, gene expression indicative of progressive organ differentiation is disrupted and RNA transcription is globally reduced. Ssrp1a-deficient embryos exhibit DNA synthesis defects and prolonged S phase, uncovering a role distinct from that of Spt16, which promotes G1 phase progression. Gene deletion/replacement experiments in Drosophila show that Ssrp1b, Ssrp1a and N-terminal Ssrp1a, equivalent to the yeast homologue Pob3, can substitute Drosophila Ssrp function. These data suggest that (1) Ssrp1b does not compensate for Ssrp1a loss in the zebrafish embryo, probably owing to insufficient expression levels, and (2) despite fundamental structural differences, the mechanisms mediating DNA accessibility by FACT are conserved between yeast and metazoans. We propose that the essential functions of Ssrp1a in DNA replication and gene transcription, together with its dynamic spatiotemporal expression, ensure organ-specific differentiation and proportional growth, which are crucial for the forming embryo.

KW - Animals

KW - Cell Cycle

KW - Cell Proliferation

KW - Chromatin Assembly and Disassembly

KW - DNA Replication

KW - DNA-Binding Proteins

KW - Drosophila

KW - Drosophila Proteins

KW - Embryo, Nonmammalian

KW - Endoderm

KW - Eye

KW - Female

KW - Gene Expression Regulation, Developmental

KW - High Mobility Group Proteins

KW - Imaginal Discs

KW - Liver

KW - Male

KW - Mitotic Index

KW - Mutation

KW - Organogenesis

KW - RNA

KW - Transcription, Genetic

KW - Transcriptional Elongation Factors

KW - Zebrafish

KW - Zebrafish Proteins

U2 - 10.1242/dev.093583

DO - 10.1242/dev.093583

M3 - Journal article

C2 - 23515471

SN - 0950-1991

VL - 140

SP - 1912

EP - 1918

JO - Development (Cambridge, England)

JF - Development (Cambridge, England)

IS - 9

ER -

Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis

Abstract

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