Spontaneous and cytokine induced basophil adhesion evaluated by microtiter assay

Sha Quan; Lars K Poulsen; Claus Michael Reimert; Christian Glue; Anders Millner; Bettina M Jensen; Tan Jinquan; Per Stahl Skov

Spontaneous and cytokine induced basophil adhesion evaluated by microtiter assay

Sha Quan, Lars K Poulsen, Claus Michael Reimert, Christian Glue, Anders Millner, Bettina M Jensen, Tan Jinquan, Per Stahl Skov

5 Citationer (Scopus)

Abstract

We have developed a microtiter assay for evaluating basophil spontaneous adhesion to extracellular matrix (ECM) proteins exemplified by fibronectin and cytokine induced basophil adhesion to bovine serum albumin (BSA). The percentage of basophils adhering to either ECM or BSA was quantified by the histamine content of the adhering basophils. The spontaneous adhesion to fibronectin was higher than to laminin and collagen type I. Both spontaneous adhesion to fibronectin and interleukin-3 (IL-3), interleukin-5 (IL-5), granulocyte/macrophage colony stimulating factor (GM-CSF) induced adhesion to BSA increased with time between 5 and 45 min. The histamine release in both spontaneous and induced basophil adhesion was lower than 3.1%. This microtiter assay is simple and reproducible and can be applied for basic and clinical studies using a limited number of partially purified basophils.

Originalsprog	Engelsk
Tidsskrift	Journal of Immunological Methods
Vol/bind	262
Udgave nummer	1-2
Sider (fra-til)	121-7
Antal sider	7
ISSN	0022-1759
Status	Udgivet - 2002

Citationsformater

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title = "Spontaneous and cytokine induced basophil adhesion evaluated by microtiter assay",

abstract = "We have developed a microtiter assay for evaluating basophil spontaneous adhesion to extracellular matrix (ECM) proteins exemplified by fibronectin and cytokine induced basophil adhesion to bovine serum albumin (BSA). The percentage of basophils adhering to either ECM or BSA was quantified by the histamine content of the adhering basophils. The spontaneous adhesion to fibronectin was higher than to laminin and collagen type I. Both spontaneous adhesion to fibronectin and interleukin-3 (IL-3), interleukin-5 (IL-5), granulocyte/macrophage colony stimulating factor (GM-CSF) induced adhesion to BSA increased with time between 5 and 45 min. The histamine release in both spontaneous and induced basophil adhesion was lower than 3.1%. This microtiter assay is simple and reproducible and can be applied for basic and clinical studies using a limited number of partially purified basophils.",

author = "Sha Quan and Poulsen, {Lars K} and Reimert, {Claus Michael} and Christian Glue and Anders Millner and Jensen, {Bettina M} and Tan Jinquan and {Stahl Skov}, Per",

year = "2002",

language = "English",

volume = "262",

pages = "121--7",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Spontaneous and cytokine induced basophil adhesion evaluated by microtiter assay

AU - Quan, Sha

AU - Poulsen, Lars K

AU - Reimert, Claus Michael

AU - Glue, Christian

AU - Millner, Anders

AU - Jensen, Bettina M

AU - Jinquan, Tan

AU - Stahl Skov, Per

PY - 2002

Y1 - 2002

N2 - We have developed a microtiter assay for evaluating basophil spontaneous adhesion to extracellular matrix (ECM) proteins exemplified by fibronectin and cytokine induced basophil adhesion to bovine serum albumin (BSA). The percentage of basophils adhering to either ECM or BSA was quantified by the histamine content of the adhering basophils. The spontaneous adhesion to fibronectin was higher than to laminin and collagen type I. Both spontaneous adhesion to fibronectin and interleukin-3 (IL-3), interleukin-5 (IL-5), granulocyte/macrophage colony stimulating factor (GM-CSF) induced adhesion to BSA increased with time between 5 and 45 min. The histamine release in both spontaneous and induced basophil adhesion was lower than 3.1%. This microtiter assay is simple and reproducible and can be applied for basic and clinical studies using a limited number of partially purified basophils.

AB - We have developed a microtiter assay for evaluating basophil spontaneous adhesion to extracellular matrix (ECM) proteins exemplified by fibronectin and cytokine induced basophil adhesion to bovine serum albumin (BSA). The percentage of basophils adhering to either ECM or BSA was quantified by the histamine content of the adhering basophils. The spontaneous adhesion to fibronectin was higher than to laminin and collagen type I. Both spontaneous adhesion to fibronectin and interleukin-3 (IL-3), interleukin-5 (IL-5), granulocyte/macrophage colony stimulating factor (GM-CSF) induced adhesion to BSA increased with time between 5 and 45 min. The histamine release in both spontaneous and induced basophil adhesion was lower than 3.1%. This microtiter assay is simple and reproducible and can be applied for basic and clinical studies using a limited number of partially purified basophils.

M3 - Journal article

SN - 0022-1759

VL - 262

SP - 121

EP - 127

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Spontaneous and cytokine induced basophil adhesion evaluated by microtiter assay

Abstract

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