Specific antibodies to Staphylococcus aureus  biofilm are present in serum from pigs with osteomyelitis

TY - JOUR

T1 - Specific antibodies to Staphylococcus aureus  biofilm are present in serum from pigs with osteomyelitis

AU - Jensen, Louise Kruse

AU - Jensen, Henrik Elvang

AU - Koch, Janne

AU - Bjarnsholt, Thomas

AU - Eickhardt-Dalbøge, Steffen Robert

AU - Shirtliff, Mark

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Background: The Achilles heel in osteomyelitis is that bacteria, primarily Staphylococcus aureus, grow as a biofilm in the bone lesions. Materials and Methods: In the present study, we explored the serum level of specific antibodies to S. aurues biofilm in porcine models of osteomyelitis. Results: Significantly increased levels of antibodies towards the specific biofilm antigen SA0688 were measured in serum from pigs with S. aureus-Associated acute and chronic osteomyelitis 5-7 and 10-14 days after inoculation, respectively. Simultaneously with raised antibody levels, an increase in serum interleukin 6 (IL 6) levels was also seen. Conclusion: The observed biofilm-specific antibody response represents a T-helper cell 17 (Th17) response and potentially a T-helper cell 1 (Th1) response. This is in agreement with previous studies in mice and rabbits speculating that S. aureus induces a Th1-and Th17-biased adaptive immune response, instead of a protective Th2 response, in order to evade the immune system, resulting in a chronic infection.

AB - Background: The Achilles heel in osteomyelitis is that bacteria, primarily Staphylococcus aureus, grow as a biofilm in the bone lesions. Materials and Methods: In the present study, we explored the serum level of specific antibodies to S. aurues biofilm in porcine models of osteomyelitis. Results: Significantly increased levels of antibodies towards the specific biofilm antigen SA0688 were measured in serum from pigs with S. aureus-Associated acute and chronic osteomyelitis 5-7 and 10-14 days after inoculation, respectively. Simultaneously with raised antibody levels, an increase in serum interleukin 6 (IL 6) levels was also seen. Conclusion: The observed biofilm-specific antibody response represents a T-helper cell 17 (Th17) response and potentially a T-helper cell 1 (Th1) response. This is in agreement with previous studies in mice and rabbits speculating that S. aureus induces a Th1-and Th17-biased adaptive immune response, instead of a protective Th2 response, in order to evade the immune system, resulting in a chronic infection.

M3 - Journal article

SN - 0258-851X

VL - 29

SP - 555

EP - 560

JO - In Vivo

JF - In Vivo

ER -