Species phylogeny and diversification process of Northeast Asian Pungitius revealed by AFLP and mtDNA markers

Hiroshi Takahashi; Peter Rask Møller; Sergei V. Shedko; Temirbekov Ramatulla; Sang-Rin Joen; Chun-Guang Zhang; Valentina G. Sideleva; Keisuke Takata; Harumi Sakai; Akira Goto; Mutsumi Nishida

doi:10.1016/j.ympev.2016.03.022

Species phylogeny and diversification process of Northeast Asian Pungitius revealed by AFLP and mtDNA markers

Hiroshi Takahashi, Peter Rask Møller, Sergei V. Shedko, Temirbekov Ramatulla, Sang-Rin Joen, Chun-Guang Zhang, Valentina G. Sideleva, Keisuke Takata, Harumi Sakai, Akira Goto, Mutsumi Nishida

13 Citationer (Scopus)

Abstract

Pungitius is a highly diversified genus of sticklebacks (Gasterosteidae) occurring widely in northern parts of the Northern Hemisphere. Several ecologically and genetically divergent types that are largely isolated reproductively but occasionally hybridize in sympatry have been discovered in Northeast Asia, although the taxonomy and evolutionary relationships among them remain unclear. We used amplified fragment length polymorphism (AFLP) and mitochondrial DNA (mtDNA) markers to infer phylogenies among individuals collected from sympatric and allopatric populations, including the type localities of the described species. Phylogenetic analyses based on 2683 polymorphic AFLP loci confirmed seven species, each of which (except for one entirely allopatric species P. platygaster) was clearly differentiated from one or two other sympatric species and constituted a highly supported monophyletic clade with conspecific allopatric populations. The phylogeny showed that two lineages arose early; one gave rise to two species (circumpolar species P. pungitius and Paratethys species P. platygaster) and the other to five species endemic to Northeast Asia (P. sinensis, P. tymensis, P. polyakovi, P. kaibarae, and P. bussei). The brackish-water, freshwater, and Omono types previously discovered in Japan were reidentified as P. pungitius, P. sinensis, and P. kaibarae, respectively. A marked incongruence was noted between the phylogenies of AFLP and mtDNA markers, suggesting the occasional occurrence of hybridization and mtDNA introgression among distinct species. Our results highlight that the marginal seas of Northeast Asia played a key role as barriers to or facilitators of gene flow in the evolution of species diversity of Pungitius concentrated in this region

Originalsprog	Engelsk
Tidsskrift	Molecular Phylogenetics and Evolution
Vol/bind	99
Sider (fra-til)	44-52
Antal sider	9
ISSN	1055-7903
DOI	https://doi.org/10.1016/j.ympev.2016.03.022
Status	Udgivet - 1 jun. 2016

Adgang til dokumentet

10.1016/j.ympev.2016.03.022

Citationsformater

@article{b28882e23acc4af792883dba2e5f8782,

title = "Species phylogeny and diversification process of Northeast Asian Pungitius revealed by AFLP and mtDNA markers",

abstract = "Pungitius is a highly diversified genus of sticklebacks (Gasterosteidae) occurring widely in northern parts of the Northern Hemisphere. Several ecologically and genetically divergent types that are largely isolated reproductively but occasionally hybridize in sympatry have been discovered in Northeast Asia, although the taxonomy and evolutionary relationships among them remain unclear. We used amplified fragment length polymorphism (AFLP) and mitochondrial DNA (mtDNA) markers to infer phylogenies among individuals collected from sympatric and allopatric populations, including the type localities of the described species. Phylogenetic analyses based on 2683 polymorphic AFLP loci confirmed seven species, each of which (except for one entirely allopatric species P. platygaster) was clearly differentiated from one or two other sympatric species and constituted a highly supported monophyletic clade with conspecific allopatric populations. The phylogeny showed that two lineages arose early; one gave rise to two species (circumpolar species P. pungitius and Paratethys species P. platygaster) and the other to five species endemic to Northeast Asia (P. sinensis, P. tymensis, P. polyakovi, P. kaibarae, and P. bussei). The brackish-water, freshwater, and Omono types previously discovered in Japan were reidentified as P. pungitius, P. sinensis, and P. kaibarae, respectively. A marked incongruence was noted between the phylogenies of AFLP and mtDNA markers, suggesting the occasional occurrence of hybridization and mtDNA introgression among distinct species. Our results highlight that the marginal seas of Northeast Asia played a key role as barriers to or facilitators of gene flow in the evolution of species diversity of Pungitius concentrated in this region",

author = "Hiroshi Takahashi and M{\o}ller, {Peter Rask} and Shedko, {Sergei V.} and Temirbekov Ramatulla and Sang-Rin Joen and Chun-Guang Zhang and Sideleva, {Valentina G.} and Keisuke Takata and Harumi Sakai and Akira Goto and Mutsumi Nishida",

year = "2016",

month = jun,

day = "1",

doi = "10.1016/j.ympev.2016.03.022",

language = "English",

volume = "99",

pages = "44--52",

journal = "Molecular Phylogenetics and Evolution",

issn = "1055-7903",

publisher = "Academic Press",

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TY - JOUR

T1 - Species phylogeny and diversification process of Northeast Asian Pungitius revealed by AFLP and mtDNA markers

AU - Takahashi, Hiroshi

AU - Møller, Peter Rask

AU - Shedko, Sergei V.

AU - Ramatulla, Temirbekov

AU - Joen, Sang-Rin

AU - Zhang, Chun-Guang

AU - Sideleva, Valentina G.

AU - Takata, Keisuke

AU - Sakai, Harumi

AU - Goto, Akira

AU - Nishida, Mutsumi

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Pungitius is a highly diversified genus of sticklebacks (Gasterosteidae) occurring widely in northern parts of the Northern Hemisphere. Several ecologically and genetically divergent types that are largely isolated reproductively but occasionally hybridize in sympatry have been discovered in Northeast Asia, although the taxonomy and evolutionary relationships among them remain unclear. We used amplified fragment length polymorphism (AFLP) and mitochondrial DNA (mtDNA) markers to infer phylogenies among individuals collected from sympatric and allopatric populations, including the type localities of the described species. Phylogenetic analyses based on 2683 polymorphic AFLP loci confirmed seven species, each of which (except for one entirely allopatric species P. platygaster) was clearly differentiated from one or two other sympatric species and constituted a highly supported monophyletic clade with conspecific allopatric populations. The phylogeny showed that two lineages arose early; one gave rise to two species (circumpolar species P. pungitius and Paratethys species P. platygaster) and the other to five species endemic to Northeast Asia (P. sinensis, P. tymensis, P. polyakovi, P. kaibarae, and P. bussei). The brackish-water, freshwater, and Omono types previously discovered in Japan were reidentified as P. pungitius, P. sinensis, and P. kaibarae, respectively. A marked incongruence was noted between the phylogenies of AFLP and mtDNA markers, suggesting the occasional occurrence of hybridization and mtDNA introgression among distinct species. Our results highlight that the marginal seas of Northeast Asia played a key role as barriers to or facilitators of gene flow in the evolution of species diversity of Pungitius concentrated in this region

AB - Pungitius is a highly diversified genus of sticklebacks (Gasterosteidae) occurring widely in northern parts of the Northern Hemisphere. Several ecologically and genetically divergent types that are largely isolated reproductively but occasionally hybridize in sympatry have been discovered in Northeast Asia, although the taxonomy and evolutionary relationships among them remain unclear. We used amplified fragment length polymorphism (AFLP) and mitochondrial DNA (mtDNA) markers to infer phylogenies among individuals collected from sympatric and allopatric populations, including the type localities of the described species. Phylogenetic analyses based on 2683 polymorphic AFLP loci confirmed seven species, each of which (except for one entirely allopatric species P. platygaster) was clearly differentiated from one or two other sympatric species and constituted a highly supported monophyletic clade with conspecific allopatric populations. The phylogeny showed that two lineages arose early; one gave rise to two species (circumpolar species P. pungitius and Paratethys species P. platygaster) and the other to five species endemic to Northeast Asia (P. sinensis, P. tymensis, P. polyakovi, P. kaibarae, and P. bussei). The brackish-water, freshwater, and Omono types previously discovered in Japan were reidentified as P. pungitius, P. sinensis, and P. kaibarae, respectively. A marked incongruence was noted between the phylogenies of AFLP and mtDNA markers, suggesting the occasional occurrence of hybridization and mtDNA introgression among distinct species. Our results highlight that the marginal seas of Northeast Asia played a key role as barriers to or facilitators of gene flow in the evolution of species diversity of Pungitius concentrated in this region

U2 - 10.1016/j.ympev.2016.03.022

DO - 10.1016/j.ympev.2016.03.022

M3 - Journal article

C2 - 26997522

SN - 1055-7903

VL - 99

SP - 44

EP - 52

JO - Molecular Phylogenetics and Evolution

JF - Molecular Phylogenetics and Evolution

ER -