SMV1, an extremely stable thermophilic virus platform for nanoparticle trafficking in the mammalian GI tract

TY - JOUR

T1 - SMV1, an extremely stable thermophilic virus platform for nanoparticle trafficking in the mammalian GI tract

AU - Uldahl, Kristine Buch

AU - Walk, S. T.

AU - Olshefsky, S. C.

AU - Young, M. J.

AU - Peng, Xu

PY - 2017/11

Y1 - 2017/11

N2 - Aims: Analysis of the stability and safety of Sulfolobus monocaudavirus 1 (SMV1) during passage through the mammalian GI tract. Methods and Results: A major challenge of using nano-vectors to target gut microbiome is their survival during passage through the extremely acidic and proteolytic environment of the mammalian GI tract. Here, we investigated the thermo-acidophilic archaeal virus SMV1 as a candidate therapeutic nano-vector for the distal mammalian GI tract microbiome. We investigated the anatomical distribution, vector stability and immunogenicity of this virus following oral ingestion in mice and compared these traits to the more classically used Inovirus vector M13KE. We found that SMV1 particles were highly stable under both simulated GI tract conditions (in vitro) and in mice (in vivo). Moreover, SMV1 could not be detected in tissues outside the GI tract and it elicited a nearly undetectable inflammatory response. Finally, we used human intestinal organoids (HIOs) to show that labelled SMV1 did not invade or otherwise perturb the human GI tract epithelium. Conclusion: Sulfolobus monocaudavirus 1 appeared stable and safe during passage though the mammalian GI tract. Significance and Impact of the Study: This is the first study evaluating an archaeal virus as a potential therapeutic nanoparticle delivery system and it opens new possibilities for future development of novel nanoplatforms.

AB - Aims: Analysis of the stability and safety of Sulfolobus monocaudavirus 1 (SMV1) during passage through the mammalian GI tract. Methods and Results: A major challenge of using nano-vectors to target gut microbiome is their survival during passage through the extremely acidic and proteolytic environment of the mammalian GI tract. Here, we investigated the thermo-acidophilic archaeal virus SMV1 as a candidate therapeutic nano-vector for the distal mammalian GI tract microbiome. We investigated the anatomical distribution, vector stability and immunogenicity of this virus following oral ingestion in mice and compared these traits to the more classically used Inovirus vector M13KE. We found that SMV1 particles were highly stable under both simulated GI tract conditions (in vitro) and in mice (in vivo). Moreover, SMV1 could not be detected in tissues outside the GI tract and it elicited a nearly undetectable inflammatory response. Finally, we used human intestinal organoids (HIOs) to show that labelled SMV1 did not invade or otherwise perturb the human GI tract epithelium. Conclusion: Sulfolobus monocaudavirus 1 appeared stable and safe during passage though the mammalian GI tract. Significance and Impact of the Study: This is the first study evaluating an archaeal virus as a potential therapeutic nanoparticle delivery system and it opens new possibilities for future development of novel nanoplatforms.

KW - archaeal virus

KW - bacteriophage

KW - mammalian GI tract

KW - nanoparticle

KW - thermophilic and acid-resistant virus

UR - http://www.scopus.com/inward/record.url?scp=85031106564&partnerID=8YFLogxK

U2 - 10.1111/jam.13584

DO - 10.1111/jam.13584

M3 - Journal article

C2 - 28891224

AN - SCOPUS:85031106564

SN - 1364-5072

VL - 123

SP - 1286

EP - 1297

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

IS - 5

ER -