Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system

Brian K Hammer; Sine Lo Svenningsen

doi:10.1007/978-1-60761-971-0_14

Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system

Brian K Hammer, Sine Lo Svenningsen

Biomolecular Sciences

1 Citationer (Scopus)

Abstract

The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).

Originalsprog	Engelsk
Titel	Quorum sensing : Methods and protocols
Redaktører	Kendra P. Rumbaugh
Antal sider	18
Vol/bind	692
Forlag	Humana Press
Publikationsdato	2011
Sider	189-206
Kapitel	14
ISBN (Trykt)	978-1-60761-970-3
ISBN (Elektronisk)	978-1-60761-971-0
DOI	https://doi.org/10.1007/978-1-60761-971-0_14
Status	Udgivet - 2011

Navn	Methods in Molecular Biology
ISSN	1064-3745

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10.1007/978-1-60761-971-0_14

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Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system. / Hammer, Brian K; Svenningsen, Sine Lo.

Quorum sensing: Methods and protocols. red. / Kendra P. Rumbaugh. Bind 692 Humana Press, 2011. s. 189-206 (Methods in Molecular Biology).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › peer review

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title = "Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system",

abstract = "The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).",

keywords = "Base Sequence, Binding Sites, Blotting, Northern, Computational Biology, Genes, Bacterial, Green Fluorescent Proteins, Kinetics, Mutagenesis, Site-Directed, Protein Biosynthesis, Quorum Sensing, RNA Splicing, RNA, Messenger, RNA, Untranslated, Reproducibility of Results, Vibrio cholerae",

author = "Hammer, {Brian K} and Svenningsen, {Sine Lo}",

year = "2011",

doi = "10.1007/978-1-60761-971-0_14",

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AU - Svenningsen, Sine Lo

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N2 - The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).

AB - The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).

KW - Base Sequence

KW - Binding Sites

KW - Blotting, Northern

KW - Computational Biology

KW - Genes, Bacterial

KW - Green Fluorescent Proteins

KW - Kinetics

KW - Mutagenesis, Site-Directed

KW - Protein Biosynthesis

KW - Quorum Sensing

KW - RNA Splicing

KW - RNA, Messenger

KW - RNA, Untranslated

KW - Reproducibility of Results

KW - Vibrio cholerae

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PB - Humana Press

ER -

Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system

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