Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system

TY - CHAP

T1 - Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system

AU - Hammer, Brian K

AU - Svenningsen, Sine Lo

PY - 2011

Y1 - 2011

N2 - The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).

AB - The two-component quorum sensing (QS) system, first described in the marine bacterium Vibrio harveyi and evolutionarily conserved among members of the genus Vibrio, has been best studied in the human pathogen Vibrio cholerae (1, 2). In the V. cholerae QS system, the response to the accumulation of extracellular autoinducers triggers a signaling cascade resulting in the transcription of four small regulatory RNAs (sRNAs). Our results support the model that the QS sRNAs bind to the 5' untranslated region of multiple mRNAs and alter the fate of one in a positive manner and several others in a negative manner. This mechanism ensures the proper timing of the QS response, which includes the expression of traits critical for virulence and for the formation of biofilms (2-6).

KW - Base Sequence

KW - Binding Sites

KW - Blotting, Northern

KW - Computational Biology

KW - Genes, Bacterial

KW - Green Fluorescent Proteins

KW - Kinetics

KW - Mutagenesis, Site-Directed

KW - Protein Biosynthesis

KW - Quorum Sensing

KW - RNA Splicing

KW - RNA, Messenger

KW - RNA, Untranslated

KW - Reproducibility of Results

KW - Vibrio cholerae

U2 - 10.1007/978-1-60761-971-0_14

DO - 10.1007/978-1-60761-971-0_14

M3 - Book chapter

C2 - 21031313

SN - 978-1-60761-970-3

VL - 692

T3 - Methods in Molecular Biology

SP - 189

EP - 206

BT - Quorum sensing

A2 - Rumbaugh, Kendra P.

PB - Humana Press

ER -