@article{3e6a8a30a68e11debc73000ea68e967b,
title = "Site specificity of psoralen-DNA interstrand cross-linking determined by nuclease Bal31 digestion",
abstract = "A novel method for determination of psoralen photo-cross-linking sites in double-stranded DNA is described, which is based on a pronounced inhibition of Bal31 exonuclease activity by psoralen-DNA interstrand cross-links. The results using a 51 base pair fragment of plasmid pUC19 and a 346 base pair fragment of pBR322 show that 5'-TA sequences are preferred cross-linking sites compared to 3'-TA sequences. They also indicate that sequences flanking the 5'-TA site influence the cross-linking efficiency at the site. The DNA photo-cross-linking by 4,5',8-trimethylpsoralen and 8-methoxypsoralen was analyzed, and these two psoralens showed identical site specificity. The 5'-TA preference is rationalized on the basis of the local DNA structure in terms of the pi-pi electronic interaction between the thymines and the intercalated psoralens, as well as on the base tilt angles of the DNA.",
author = "Zhen, {W P} and O Buchardt and Henrik Nielsen and Nielsen, {P E}",
note = "Keywords: Base Sequence; DNA; DNA Restriction Enzymes; Endodeoxyribonucleases; Exodeoxyribonucleases; Photochemistry; Plasmids; Psoralens; Trioxsalen",
year = "1986",
language = "English",
volume = "25",
pages = "6598--603",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "21",
}