@article{0acf36c0f2ec11dfb6d2000ea68e967b,
title = "Single-molecule denaturation mapping of DNA in nanofluidic channels",
abstract = "Here we explore the potential power of denaturation mapping as a single-molecule technique. By partially denaturing YOYO{\textregistered}-1-labeled DNA in nanofluidic channels with a combination of formamide and local heating, we obtain a sequence-dependent {"}barcode{"} corresponding to a series of local dips and peaks in the intensity trace along the extended molecule. We demonstrate that this structure arises from the physics of local denaturation: statistical mechanical calculations of sequence-dependent melting probability can predict the barcode to be observed experimentally for a given sequence. Consequently, the technique is sensitive to sequence variation without requiring enzymatic labeling or a restriction step. This technique may serve as the basis for a new mapping technology ideally suited for investigating the long-range structure of entire genomes extracted from single cells.",
author = "Walter Reisner and Larsen, {Niels B} and Asli Silahtaroglu and Anders Kristensen and Niels Tommerup and Tegenfeldt, {Jonas O} and Henrik Flyvbjerg",
note = "Keywords: Algorithms; Bacteriophages; Benzoxazoles; DNA; Formamides; Microfluidic Analytical Techniques; Models, Chemical; Nanotechnology; Nucleic Acid Conformation; Nucleic Acid Denaturation; Quinolinium Compounds; Transition Temperature",
year = "2010",
month = jul,
day = "27",
doi = "10.1073/pnas.1007081107",
language = "English",
volume = "107",
pages = "13294--9",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "30",
}