@article{2907df80ab5311ddb5e9000ea68e967b,
title = "Single-copy insertion of transgenes in Caenorhabditis elegans.",
abstract = "At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA. These transgenes are typically overexpressed in somatic cells and silenced in the germline. We have developed a method that inserts a single copy of a transgene into a defined site. Mobilization of a Mos1 transposon generates a double-strand break in noncoding DNA. The break is repaired by copying DNA from an extrachromosomal template into the chromosomal site. Homozygous single-copy insertions can be obtained in less than 2 weeks by injecting approximately 20 worms. We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines.",
author = "Christian Fr{\o}kjaer-Jensen and Davis, {M Wayne} and Hopkins, {Christopher E} and Newman, {Blake J} and Thummel, {Jason M} and S{\o}ren-Peter Olesen and Morten Grunnet and Jorgensen, {Erik M}",
year = "2008",
doi = "10.1038/ng.248",
language = "English",
volume = "40",
pages = "1375--83",
journal = "Nature: New biology",
issn = "1061-4036",
publisher = "nature publishing group",
number = "11",
}