Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor: Cell

Xun Xu; Yong Hou; Xuyang Yin; Li Bao; Aifa Tang; Luting Song; Fuqiang Li; Shirley Tsang; Kui Wu; Hanjie Wu; Weiming He; Liang Zeng; Manjie Xing; Renhua Wu; Hui Jiang; Xiao Liu; Dandan Cao; Guangwu Guo; Xueda Hu; Yaoting Gui; Zesong Li; Wenyue Xie; Xiaojuan Sun; Min Shi; Zhiming Cai; Bin Wang; Meiming Zhong; Jingxiang Li; Zuhong Lu; Ning Gu; Xiuqing Zhang; Laurie Goodman; Lars Bolund; Jian Wang; Huanming Yang; Karsten Kristiansen; Michael Dean; Yingrui Li; Jun Wang

doi:10.1016/j.cell.2012.02.025

Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor: Cell

Xun Xu, Yong Hou, Xuyang Yin, Li Bao, Aifa Tang, Luting Song, Fuqiang Li, Shirley Tsang, Kui Wu, Hanjie Wu, Weiming He, Liang Zeng, Manjie Xing, Renhua Wu, Hui Jiang, Xiao Liu, Dandan Cao, Guangwu Guo, Xueda Hu, Yaoting GuiZesong Li, Wenyue Xie, Xiaojuan Sun, Min Shi, Zhiming Cai, Bin Wang, Meiming Zhong, Jingxiang Li, Zuhong Lu, Ning Gu, Xiuqing Zhang, Laurie Goodman, Lars Bolund, Jian Wang, Huanming Yang, Karsten Kristiansen, Michael Dean, Yingrui Li, Jun Wang

Biologisk Institut

445 Citationer (Scopus)

Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer and has very few mutations that are shared between different patients. To better understand the intratumoral genetics underlying mutations of ccRCC, we carried out single-cell exome sequencing on a ccRCC tumor and its adjacent kidney tissue. Our data indicate that this tumor was unlikely to have resulted from mutations in VHL and PBRM1. Quantitative population genetic analysis indicates that the tumor did not contain any significant clonal subpopulations and also showed that mutations that had different allele frequencies within the population also had different mutation spectrums. Analyses of these data allowed us to delineate a detailed intratumoral genetic landscape at a single-cell level. Our pilot study demonstrates that ccRCC may be more genetically complex than previously thought and provides information that can lead to new ways to investigate individual tumors, with the aim of developing more effective cellular targeted therapies.

Originalsprog	Dansk
Tidsskrift	Cell
Vol/bind	148
Udgave nummer	5
Sider (fra-til)	886-895
Antal sider	10
ISSN	0092-8674
DOI	https://doi.org/10.1016/j.cell.2012.02.025
Status	Udgivet - 2 mar. 2012

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10.1016/j.cell.2012.02.025

http://www.sciencedirect.com/science/article/pii/S0092867412002255

Citationsformater

Xu, X, Hou, Y, Yin, X, Bao, L, Tang, A, Song, L, Li, F, Tsang, S, Wu, K, Wu, H, He, W, Zeng, L, Xing, M, Wu, R, Jiang, H, Liu, X, Cao, D, Guo, G, Hu, X, Gui, Y, Li, Z, Xie, W, Sun, X, Shi, M, Cai, Z, Wang, B, Zhong, M, Li, J, Lu, Z, Gu, N, Zhang, X, Goodman, L, Bolund, L, Wang, J, Yang, H, Kristiansen, K, Dean, M, Li, Y & Wang, J 2012, 'Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor: Cell', Cell, bind 148, nr. 5, s. 886-895. https://doi.org/10.1016/j.cell.2012.02.025

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title = "Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor: Cell",

abstract = "Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer and has very few mutations that are shared between different patients. To better understand the intratumoral genetics underlying mutations of ccRCC, we carried out single-cell exome sequencing on a ccRCC tumor and its adjacent kidney tissue. Our data indicate that this tumor was unlikely to have resulted from mutations in VHL and PBRM1. Quantitative population genetic analysis indicates that the tumor did not contain any significant clonal subpopulations and also showed that mutations that had different allele frequencies within the population also had different mutation spectrums. Analyses of these data allowed us to delineate a detailed intratumoral genetic landscape at a single-cell level. Our pilot study demonstrates that ccRCC may be more genetically complex than previously thought and provides information that can lead to new ways to investigate individual tumors, with the aim of developing more effective cellular targeted therapies.",

author = "Xun Xu and Yong Hou and Xuyang Yin and Li Bao and Aifa Tang and Luting Song and Fuqiang Li and Shirley Tsang and Kui Wu and Hanjie Wu and Weiming He and Liang Zeng and Manjie Xing and Renhua Wu and Hui Jiang and Xiao Liu and Dandan Cao and Guangwu Guo and Xueda Hu and Yaoting Gui and Zesong Li and Wenyue Xie and Xiaojuan Sun and Min Shi and Zhiming Cai and Bin Wang and Meiming Zhong and Jingxiang Li and Zuhong Lu and Ning Gu and Xiuqing Zhang and Laurie Goodman and Lars Bolund and Jian Wang and Huanming Yang and Karsten Kristiansen and Michael Dean and Yingrui Li and Jun Wang",

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T1 - Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor

T2 - Cell

AU - Xu, Xun

AU - Hou, Yong

AU - Yin, Xuyang

AU - Bao, Li

AU - Tang, Aifa

AU - Song, Luting

AU - Li, Fuqiang

AU - Tsang, Shirley

AU - Wu, Kui

AU - Wu, Hanjie

AU - He, Weiming

AU - Zeng, Liang

AU - Xing, Manjie

AU - Wu, Renhua

AU - Jiang, Hui

AU - Liu, Xiao

AU - Cao, Dandan

AU - Guo, Guangwu

AU - Hu, Xueda

AU - Gui, Yaoting

AU - Li, Zesong

AU - Xie, Wenyue

AU - Sun, Xiaojuan

AU - Shi, Min

AU - Cai, Zhiming

AU - Wang, Bin

AU - Zhong, Meiming

AU - Li, Jingxiang

AU - Lu, Zuhong

AU - Gu, Ning

AU - Zhang, Xiuqing

AU - Goodman, Laurie

AU - Bolund, Lars

AU - Wang, Jian

AU - Yang, Huanming

AU - Kristiansen, Karsten

AU - Dean, Michael

AU - Li, Yingrui

AU - Wang, Jun

PY - 2012/3/2

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N2 - Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer and has very few mutations that are shared between different patients. To better understand the intratumoral genetics underlying mutations of ccRCC, we carried out single-cell exome sequencing on a ccRCC tumor and its adjacent kidney tissue. Our data indicate that this tumor was unlikely to have resulted from mutations in VHL and PBRM1. Quantitative population genetic analysis indicates that the tumor did not contain any significant clonal subpopulations and also showed that mutations that had different allele frequencies within the population also had different mutation spectrums. Analyses of these data allowed us to delineate a detailed intratumoral genetic landscape at a single-cell level. Our pilot study demonstrates that ccRCC may be more genetically complex than previously thought and provides information that can lead to new ways to investigate individual tumors, with the aim of developing more effective cellular targeted therapies.

AB - Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer and has very few mutations that are shared between different patients. To better understand the intratumoral genetics underlying mutations of ccRCC, we carried out single-cell exome sequencing on a ccRCC tumor and its adjacent kidney tissue. Our data indicate that this tumor was unlikely to have resulted from mutations in VHL and PBRM1. Quantitative population genetic analysis indicates that the tumor did not contain any significant clonal subpopulations and also showed that mutations that had different allele frequencies within the population also had different mutation spectrums. Analyses of these data allowed us to delineate a detailed intratumoral genetic landscape at a single-cell level. Our pilot study demonstrates that ccRCC may be more genetically complex than previously thought and provides information that can lead to new ways to investigate individual tumors, with the aim of developing more effective cellular targeted therapies.

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DO - 10.1016/j.cell.2012.02.025

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