Simvastatin and atorvastatin reduce the mechanical properties of tendon constructs in vitro and introduce catabolic changes in the gene expression pattern

Pernilla Eliasson; Rene B Svensson; Antonis Giannopoulos; Christian Eismark; Michael Kjær; Peter Schjerling; Katja M Heinemeier

doi:10.1371/journal.pone.0172797

Simvastatin and atorvastatin reduce the mechanical properties of tendon constructs in vitro and introduce catabolic changes in the gene expression pattern

Pernilla Eliasson, Rene B Svensson, Antonis Giannopoulos, Christian Eismark, Michael Kjær, Peter Schjerling, Katja M Heinemeier

5 Citationer (Scopus)

56 Downloads (Pure)

Abstract

Treatment with lipid-lowering drugs, statins, is common all over the world. Lately, the occurrence of spontaneous tendon ruptures or tendinosis have suggested a negative influence of statins upon tendon tissue. But how statins might influence tendons is not clear. In the present study, we investigated the effect of statin treatment on mechanical strength, cell proliferation, collagen content and gene expression pattern in a tendon-like tissue made from human tenocytes in vitro. Human tendon fibroblasts were grown in a 3D tissue culture model (tendon constructs), and treated with either simvastatin or atorvastatin, low or high dose, respectively, for up to seven days. After seven days of treatment, mechanical testing of the constructs was performed. Collagen content and cell proliferation were also determined. mRNA levels of several target genes were measured after one or seven days. The maximum force and stiffness were reduced by both statins after 7 days (p<0.05), while the cross sectional area was unaffected. Further, the collagen content was reduced by atorvastatin (p = 0.01) and the cell proliferation rate was decreased by both types of statins (p<0.05). Statin treatment also introduced increased mRNA levels of MMP-1, MMP-3, MMP-13, TIMP-1 and decreased levels of collagen type 1 and 3. In conclusion, statin treatment appears to have a negative effect on tendon matrix quality as seen by a reduced strength of the tendon constructs. Further, activated catabolic changes in the gene expression pattern and a reduced collagen content indicated a disturbed balance in matrix production of tendon due to statin administration.

Originalsprog	Engelsk
Artikelnummer	e0172797
Tidsskrift	PLOS ONE
Vol/bind	12
Udgave nummer	3
Antal sider	16
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0172797
Status	Udgivet - 1 mar. 2017

Adgang til dokumentet

10.1371/journal.pone.0172797Licens: CC BY

Simvastatin and atorvastatin reduce the mechanical properties of tendon constructs in vitro and introduce catabolic changes in the gene expression patternForlagets udgivne version, 1,33 MBLicens: CC BY

Citationsformater

@article{2c9a3f288c53413cb840e6e179fbadba,

title = "Simvastatin and atorvastatin reduce the mechanical properties of tendon constructs in vitro and introduce catabolic changes in the gene expression pattern",

abstract = "Treatment with lipid-lowering drugs, statins, is common all over the world. Lately, the occurrence of spontaneous tendon ruptures or tendinosis have suggested a negative influence of statins upon tendon tissue. But how statins might influence tendons is not clear. In the present study, we investigated the effect of statin treatment on mechanical strength, cell proliferation, collagen content and gene expression pattern in a tendon-like tissue made from human tenocytes in vitro. Human tendon fibroblasts were grown in a 3D tissue culture model (tendon constructs), and treated with either simvastatin or atorvastatin, low or high dose, respectively, for up to seven days. After seven days of treatment, mechanical testing of the constructs was performed. Collagen content and cell proliferation were also determined. mRNA levels of several target genes were measured after one or seven days. The maximum force and stiffness were reduced by both statins after 7 days (p<0.05), while the cross sectional area was unaffected. Further, the collagen content was reduced by atorvastatin (p = 0.01) and the cell proliferation rate was decreased by both types of statins (p<0.05). Statin treatment also introduced increased mRNA levels of MMP-1, MMP-3, MMP-13, TIMP-1 and decreased levels of collagen type 1 and 3. In conclusion, statin treatment appears to have a negative effect on tendon matrix quality as seen by a reduced strength of the tendon constructs. Further, activated catabolic changes in the gene expression pattern and a reduced collagen content indicated a disturbed balance in matrix production of tendon due to statin administration.",

keywords = "Adolescent, Adult, Atorvastatin Calcium, Cell Proliferation, Cells, Cultured, Collagen, Energy Metabolism, Fibroblasts, Gene Expression Regulation, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Matrix Metalloproteinases, Mechanical Phenomena, Simvastatin, Tendon Injuries, Tendons, Tissue Inhibitor of Metalloproteinase-1, Young Adult, Journal Article",

author = "Pernilla Eliasson and Svensson, {Rene B} and Antonis Giannopoulos and Christian Eismark and Michael Kj{\ae}r and Peter Schjerling and Heinemeier, {Katja M}",

year = "2017",

month = mar,

day = "1",

doi = "10.1371/journal.pone.0172797",

language = "English",

volume = "12",

journal = "PLoS Computational Biology",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "3",

}

TY - JOUR

T1 - Simvastatin and atorvastatin reduce the mechanical properties of tendon constructs in vitro and introduce catabolic changes in the gene expression pattern

AU - Eliasson, Pernilla

AU - Svensson, Rene B

AU - Giannopoulos, Antonis

AU - Eismark, Christian

AU - Kjær, Michael

AU - Schjerling, Peter

AU - Heinemeier, Katja M

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Treatment with lipid-lowering drugs, statins, is common all over the world. Lately, the occurrence of spontaneous tendon ruptures or tendinosis have suggested a negative influence of statins upon tendon tissue. But how statins might influence tendons is not clear. In the present study, we investigated the effect of statin treatment on mechanical strength, cell proliferation, collagen content and gene expression pattern in a tendon-like tissue made from human tenocytes in vitro. Human tendon fibroblasts were grown in a 3D tissue culture model (tendon constructs), and treated with either simvastatin or atorvastatin, low or high dose, respectively, for up to seven days. After seven days of treatment, mechanical testing of the constructs was performed. Collagen content and cell proliferation were also determined. mRNA levels of several target genes were measured after one or seven days. The maximum force and stiffness were reduced by both statins after 7 days (p<0.05), while the cross sectional area was unaffected. Further, the collagen content was reduced by atorvastatin (p = 0.01) and the cell proliferation rate was decreased by both types of statins (p<0.05). Statin treatment also introduced increased mRNA levels of MMP-1, MMP-3, MMP-13, TIMP-1 and decreased levels of collagen type 1 and 3. In conclusion, statin treatment appears to have a negative effect on tendon matrix quality as seen by a reduced strength of the tendon constructs. Further, activated catabolic changes in the gene expression pattern and a reduced collagen content indicated a disturbed balance in matrix production of tendon due to statin administration.

AB - Treatment with lipid-lowering drugs, statins, is common all over the world. Lately, the occurrence of spontaneous tendon ruptures or tendinosis have suggested a negative influence of statins upon tendon tissue. But how statins might influence tendons is not clear. In the present study, we investigated the effect of statin treatment on mechanical strength, cell proliferation, collagen content and gene expression pattern in a tendon-like tissue made from human tenocytes in vitro. Human tendon fibroblasts were grown in a 3D tissue culture model (tendon constructs), and treated with either simvastatin or atorvastatin, low or high dose, respectively, for up to seven days. After seven days of treatment, mechanical testing of the constructs was performed. Collagen content and cell proliferation were also determined. mRNA levels of several target genes were measured after one or seven days. The maximum force and stiffness were reduced by both statins after 7 days (p<0.05), while the cross sectional area was unaffected. Further, the collagen content was reduced by atorvastatin (p = 0.01) and the cell proliferation rate was decreased by both types of statins (p<0.05). Statin treatment also introduced increased mRNA levels of MMP-1, MMP-3, MMP-13, TIMP-1 and decreased levels of collagen type 1 and 3. In conclusion, statin treatment appears to have a negative effect on tendon matrix quality as seen by a reduced strength of the tendon constructs. Further, activated catabolic changes in the gene expression pattern and a reduced collagen content indicated a disturbed balance in matrix production of tendon due to statin administration.

KW - Adolescent

KW - Adult

KW - Atorvastatin Calcium

KW - Cell Proliferation

KW - Cells, Cultured

KW - Collagen

KW - Energy Metabolism

KW - Fibroblasts

KW - Gene Expression Regulation

KW - Humans

KW - Hydroxymethylglutaryl-CoA Reductase Inhibitors

KW - Matrix Metalloproteinases

KW - Mechanical Phenomena

KW - Simvastatin

KW - Tendon Injuries

KW - Tendons

KW - Tissue Inhibitor of Metalloproteinase-1

KW - Young Adult

KW - Journal Article

U2 - 10.1371/journal.pone.0172797

DO - 10.1371/journal.pone.0172797

M3 - Journal article

C2 - 28264197

SN - 1932-6203

VL - 12

JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 3

M1 - e0172797

ER -

Simvastatin and atorvastatin reduce the mechanical properties of tendon constructs in vitro and introduce catabolic changes in the gene expression pattern

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater