Signaling cascades: consequences of varying substrate and phosphatase levels

Elisenda Feliu, Michael Knudsen, Carsten Wiuf

3 Citationer (Scopus)

Abstract

We study signaling cascades with an arbitrary number of layers of one-site phosphorylation cycles. Such cascades are abundant in nature and integrated parts of many pathways. Based on the Michaelis–Menten model of enzyme kinetics and the law of mass-action, we derive explicit analytic expressions for how the steady state concentrations and the total amounts of substrates, kinase, and phosphatates depend on each other. In particular, we use these to study how the responses (the activated substrates) vary as a function of the available amounts of substrates, kinase, and phosphatases. Our results provide insight into how the cascade response is affected by crosstalk and external regulation.
OriginalsprogEngelsk
BogserieAdvances in Experimental Medicine and Biology
Vol/bind736
Sider (fra-til)81-94
Antal sider14
ISSN0065-2598
DOI
StatusUdgivet - 2012

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