Setting up Schizosaccharomyces pombe crosses/matings

TY - JOUR

T1 - Setting up Schizosaccharomyces pombe crosses/matings

AU - Ekwall, Karl

AU - Thon, Genevieve

PY - 2017/7

Y1 - 2017/7

N2 - Here we provide methods for setting up standard crosses with Schizosaccharomyces pombe strains. All strain genotypes and pedigrees should be recorded in a laboratory strain book. Matings between two haploid strains of interest are induced on solid medium poor in nitrogen. Usually, sporulation agar (SPA) plates are preferred, but for difficult matings it is advisable to try several mating media in parallel because one medium might allow for more efficient mating. Protoplast fusion can be used to produce zygotes from sterile mutants that fail to mate.

AB - Here we provide methods for setting up standard crosses with Schizosaccharomyces pombe strains. All strain genotypes and pedigrees should be recorded in a laboratory strain book. Matings between two haploid strains of interest are induced on solid medium poor in nitrogen. Usually, sporulation agar (SPA) plates are preferred, but for difficult matings it is advisable to try several mating media in parallel because one medium might allow for more efficient mating. Protoplast fusion can be used to produce zygotes from sterile mutants that fail to mate.

UR - http://www.scopus.com/inward/record.url?scp=85021759545&partnerID=8YFLogxK

U2 - 10.1101/pdb.prot091694

DO - 10.1101/pdb.prot091694

M3 - Journal article

C2 - 28679701

AN - SCOPUS:85021759545

SN - 1940-3402

VL - 2017

SP - 533

EP - 536

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 7

ER -