Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis

TY - JOUR

T1 - Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis

AU - Morgan, Philip E

AU - Sturgess, Allan D

AU - Hennessy, Annemarie

AU - Davies, Michael Jonathan

PY - 2007/12

Y1 - 2007/12

N2 - Increased oxidative stress is a hallmark of the autoimmune disease systemic lupus erythematosus (SLE). This study compares serum protein oxidation levels in SLE patients without and with renal involvement (lupus nephritis); the latter have a significantly poorer prognosis. Similar increases in protein carbonyls and decreases in protein thiols were observed in both SLE groups compared to controls. Protein carbonyl distribution, determined by Western blotting of 2D gels, was similar in both SLE groups, suggesting factors other than oxidation also play a role in SLE complications. 2D electrophoresis examined the serum proteome further. Six proteins were significantly decreases in non-renal SLE patients compared to controls; five were identified by mass spectrometry, including one isoform of pro-atherogenic apoCIII. Total apoCIII levels (assessed by ELISA) in lupus nephritis patients were significantly elevated compared to controls or non-renal SLE patients. Thus, levels of oxidized proteins and apoCIII may be useful biomarkers in SLE studies.

AB - Increased oxidative stress is a hallmark of the autoimmune disease systemic lupus erythematosus (SLE). This study compares serum protein oxidation levels in SLE patients without and with renal involvement (lupus nephritis); the latter have a significantly poorer prognosis. Similar increases in protein carbonyls and decreases in protein thiols were observed in both SLE groups compared to controls. Protein carbonyl distribution, determined by Western blotting of 2D gels, was similar in both SLE groups, suggesting factors other than oxidation also play a role in SLE complications. 2D electrophoresis examined the serum proteome further. Six proteins were significantly decreases in non-renal SLE patients compared to controls; five were identified by mass spectrometry, including one isoform of pro-atherogenic apoCIII. Total apoCIII levels (assessed by ELISA) in lupus nephritis patients were significantly elevated compared to controls or non-renal SLE patients. Thus, levels of oxidized proteins and apoCIII may be useful biomarkers in SLE studies.

KW - Adult

KW - Apolipoprotein C-III

KW - Biological Markers

KW - Blood Proteins

KW - Electrophoresis, Gel, Two-Dimensional

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - Lupus Erythematosus, Systemic

KW - Male

KW - Middle Aged

KW - Nephritis

KW - Oxidation-Reduction

KW - Proteome

U2 - 10.1080/10715760701684809

DO - 10.1080/10715760701684809

M3 - Journal article

C2 - 17957542

SN - 1071-5762

VL - 41

SP - 1301

EP - 1312

JO - Free Radical Research

JF - Free Radical Research

IS - 12

ER -