Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection

Andreas Munk Petersen; Christian Schou; Hengameh Mirsepasi; Jørgen Engberg; Alice Friis-Møller; Inge Nordgaard-Lassen; Signe Wildt; Karen Angeliki Krogfelt

doi:10.3109/00365521.2011.639080

Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection

Andreas Munk Petersen, Christian Schou, Hengameh Mirsepasi, Jørgen Engberg, Alice Friis-Møller, Inge Nordgaard-Lassen, Signe Wildt, Karen Angeliki Krogfelt

7 Citationer (Scopus)

Abstract

Background. Several serologic tests, including anti-outer membrane porin C antibody (Omp C), are used for screening and as marker of disease course in inflammatory bowel diseases (IBD). Our aim was to investigate possible differences in Omp C level in patients with active and inactive IBD compared to controls. Methods. All blood samples were tested for Omp C. Disease activity was evaluated by Harvey Bradshaw Index, Simple Clinical Activity Index and Modified Pouchitis Disease Activity Index. Results. Blood samples were collected from 113 patients and 60 controls. Patients with active IBD did not have a higher level of Omp C than patients in remission. Surprisingly, in patients with active Crohn's disease a significantly lower level of Omp C was found compared with patients with inactive Crohn's disease (p < 0.05). All other groups among patients with IBD did have a significantly higher level of Omp C, compared with controls, including patients with acute gastroenteritis (p < 0.05). Although IBD patients with phylogroup B2 E. coli cultured from their fecal samples, were more likely to have a positive Omp C test (p < 0.05), this could not explain the low Omp C level in the subgroup of patients with active Crohn's disease. Conclusions. Omp C titer was not raised in patients with active IBD compared with patients in remission. In addition, there was no difference in Omp C level in patients with active Crohn's disease compared with controls. These observations do not support the use of Omp C serology testing, either in disease activity assessment, or in screening for active Crohn's disease.

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Gastroenterology
Vol/bind	47
Udgave nummer	2
Sider (fra-til)	155-61
Antal sider	7
ISSN	0036-5521
DOI	https://doi.org/10.3109/00365521.2011.639080
Status	Udgivet - feb. 2012

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10.3109/00365521.2011.639080

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Petersen, A. M., Schou, C., Mirsepasi, H., Engberg, J., Friis-Møller, A., Nordgaard-Lassen, I., Wildt, S., & Krogfelt, K. A. (2012). Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection. Scandinavian Journal of Gastroenterology, 47(2), 155-61. https://doi.org/10.3109/00365521.2011.639080

Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection. / Petersen, Andreas Munk; Schou, Christian; Mirsepasi, Hengameh et al.

I: Scandinavian Journal of Gastroenterology, Bind 47, Nr. 2, 02.2012, s. 155-61.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Petersen, AM, Schou, C, Mirsepasi, H, Engberg, J, Friis-Møller, A, Nordgaard-Lassen, I, Wildt, S & Krogfelt, KA 2012, 'Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection', Scandinavian Journal of Gastroenterology, bind 47, nr. 2, s. 155-61. https://doi.org/10.3109/00365521.2011.639080

@article{c574b00f68934bcfa58257e9a159e607,

title = "Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection",

abstract = "Background. Several serologic tests, including anti-outer membrane porin C antibody (Omp C), are used for screening and as marker of disease course in inflammatory bowel diseases (IBD). Our aim was to investigate possible differences in Omp C level in patients with active and inactive IBD compared to controls. Methods. All blood samples were tested for Omp C. Disease activity was evaluated by Harvey Bradshaw Index, Simple Clinical Activity Index and Modified Pouchitis Disease Activity Index. Results. Blood samples were collected from 113 patients and 60 controls. Patients with active IBD did not have a higher level of Omp C than patients in remission. Surprisingly, in patients with active Crohn's disease a significantly lower level of Omp C was found compared with patients with inactive Crohn's disease (p < 0.05). All other groups among patients with IBD did have a significantly higher level of Omp C, compared with controls, including patients with acute gastroenteritis (p < 0.05). Although IBD patients with phylogroup B2 E. coli cultured from their fecal samples, were more likely to have a positive Omp C test (p < 0.05), this could not explain the low Omp C level in the subgroup of patients with active Crohn's disease. Conclusions. Omp C titer was not raised in patients with active IBD compared with patients in remission. In addition, there was no difference in Omp C level in patients with active Crohn's disease compared with controls. These observations do not support the use of Omp C serology testing, either in disease activity assessment, or in screening for active Crohn's disease.",

keywords = "Acute Disease, Adolescent, Adult, Aged, Aged, 80 and over, Antibodies, Bacterial/blood, Bacterial Outer Membrane Proteins/immunology, Child, Chronic Disease, Colitis, Ulcerative/blood, Colonic Pouches/pathology, Crohn Disease/blood, Diarrhea/blood, Dysentery/blood, Escherichia coli/immunology, Escherichia coli Infections/complications, Escherichia coli Proteins/immunology, Female, Gastroenteritis/blood, Humans, Male, Middle Aged, Porins/immunology, Pouchitis/blood, Sensitivity and Specificity, Serologic Tests, Young Adult",

author = "Petersen, {Andreas Munk} and Christian Schou and Hengameh Mirsepasi and J{\o}rgen Engberg and Alice Friis-M{\o}ller and Inge Nordgaard-Lassen and Signe Wildt and Krogfelt, {Karen Angeliki}",

year = "2012",

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doi = "10.3109/00365521.2011.639080",

language = "English",

volume = "47",

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journal = "Scandinavian Journal of Gastroenterology",

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TY - JOUR

T1 - Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection

AU - Petersen, Andreas Munk

AU - Schou, Christian

AU - Mirsepasi, Hengameh

AU - Engberg, Jørgen

AU - Friis-Møller, Alice

AU - Nordgaard-Lassen, Inge

AU - Wildt, Signe

AU - Krogfelt, Karen Angeliki

PY - 2012/2

Y1 - 2012/2

N2 - Background. Several serologic tests, including anti-outer membrane porin C antibody (Omp C), are used for screening and as marker of disease course in inflammatory bowel diseases (IBD). Our aim was to investigate possible differences in Omp C level in patients with active and inactive IBD compared to controls. Methods. All blood samples were tested for Omp C. Disease activity was evaluated by Harvey Bradshaw Index, Simple Clinical Activity Index and Modified Pouchitis Disease Activity Index. Results. Blood samples were collected from 113 patients and 60 controls. Patients with active IBD did not have a higher level of Omp C than patients in remission. Surprisingly, in patients with active Crohn's disease a significantly lower level of Omp C was found compared with patients with inactive Crohn's disease (p < 0.05). All other groups among patients with IBD did have a significantly higher level of Omp C, compared with controls, including patients with acute gastroenteritis (p < 0.05). Although IBD patients with phylogroup B2 E. coli cultured from their fecal samples, were more likely to have a positive Omp C test (p < 0.05), this could not explain the low Omp C level in the subgroup of patients with active Crohn's disease. Conclusions. Omp C titer was not raised in patients with active IBD compared with patients in remission. In addition, there was no difference in Omp C level in patients with active Crohn's disease compared with controls. These observations do not support the use of Omp C serology testing, either in disease activity assessment, or in screening for active Crohn's disease.

AB - Background. Several serologic tests, including anti-outer membrane porin C antibody (Omp C), are used for screening and as marker of disease course in inflammatory bowel diseases (IBD). Our aim was to investigate possible differences in Omp C level in patients with active and inactive IBD compared to controls. Methods. All blood samples were tested for Omp C. Disease activity was evaluated by Harvey Bradshaw Index, Simple Clinical Activity Index and Modified Pouchitis Disease Activity Index. Results. Blood samples were collected from 113 patients and 60 controls. Patients with active IBD did not have a higher level of Omp C than patients in remission. Surprisingly, in patients with active Crohn's disease a significantly lower level of Omp C was found compared with patients with inactive Crohn's disease (p < 0.05). All other groups among patients with IBD did have a significantly higher level of Omp C, compared with controls, including patients with acute gastroenteritis (p < 0.05). Although IBD patients with phylogroup B2 E. coli cultured from their fecal samples, were more likely to have a positive Omp C test (p < 0.05), this could not explain the low Omp C level in the subgroup of patients with active Crohn's disease. Conclusions. Omp C titer was not raised in patients with active IBD compared with patients in remission. In addition, there was no difference in Omp C level in patients with active Crohn's disease compared with controls. These observations do not support the use of Omp C serology testing, either in disease activity assessment, or in screening for active Crohn's disease.

KW - Acute Disease

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Antibodies, Bacterial/blood

KW - Bacterial Outer Membrane Proteins/immunology

KW - Child

KW - Chronic Disease

KW - Colitis, Ulcerative/blood

KW - Colonic Pouches/pathology

KW - Crohn Disease/blood

KW - Diarrhea/blood

KW - Dysentery/blood

KW - Escherichia coli/immunology

KW - Escherichia coli Infections/complications

KW - Escherichia coli Proteins/immunology

KW - Female

KW - Gastroenteritis/blood

KW - Humans

KW - Male

KW - Middle Aged

KW - Porins/immunology

KW - Pouchitis/blood

KW - Sensitivity and Specificity

KW - Serologic Tests

KW - Young Adult

U2 - 10.3109/00365521.2011.639080

DO - 10.3109/00365521.2011.639080

M3 - Journal article

C2 - 22150030

SN - 0036-5521

VL - 47

SP - 155

EP - 161

JO - Scandinavian Journal of Gastroenterology

JF - Scandinavian Journal of Gastroenterology

IS - 2

ER -

Seroreactivity to E. coli outer membrane protein C antibodies in active inflammatory bowel disease; diagnostic value and correlation with phylogroup B2 E. coli infection

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