Sequence organization of barley centromeres.

TY - JOUR

T1 - Sequence organization of barley centromeres.

AU - Hudakova, S

AU - Michalek, W

AU - Presting, G G

AU - ten Hoopen, R

AU - dos Santos, K

AU - Jasencakova, Zusana

AU - Schubert, I

N1 - Keywords: Blotting, Southern; Centromere; Cloning, Molecular; DNA Restriction Enzymes; DNA, Plant; Hordeum; In Situ Hybridization, Fluorescence; Molecular Sequence Data; Mutagenesis, Insertional; Retroelements; Sequence Analysis, DNA

PY - 2001

Y1 - 2001

N2 - By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of approximately 7 kb, arranged in tandem, include long terminal repeats (LTRs) (approximately 1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (approximately 200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.

AB - By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of approximately 7 kb, arranged in tandem, include long terminal repeats (LTRs) (approximately 1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (approximately 200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.

M3 - Journal article

C2 - 11812833

SN - 0305-1048

VL - 29

SP - 5029

EP - 5035

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 24

ER -