Sequence analysis of 17 NRXN1 deletions

TY - JOUR

T1 - Sequence analysis of 17 NRXN1 deletions

AU - Hoeffding, Louise Kristine Enggaard

AU - Hansen, Thomas

AU - Ingason, Andrés

AU - Doung, Linh

AU - Thygesen, Johan H

AU - Møller, Rikke S

AU - Tommerup, Niels

AU - Kirov, George

AU - Rujescu, Dan

AU - Larsen, Lars A

AU - Werge, Thomas

N1 - © 2013 Wiley Periodicals, Inc.

PY - 2014/1

Y1 - 2014/1

N2 - Background: Genome instability plays fundamental roles in human evolution and phenotypic variation within our population. This instability leads to genomic rearrangements that are involved in a wide variety of human disorders, including congenital and neurodevelopmental disorders, and cancers. Insight into the molecular mechanisms governing such genomic rearrangements may increase our understanding of disease pathology and evolutionary processes. Here we analyse 17 carriers of non-recurrent deletions in the NRXN1 gene, which have been associated with neurodevelopmental disorders, e.g. schizophrenia, autism and epilepsies. Methods: 17 non-recurrent NRXN1 deletions identified by GWA were sequenced to map the breakpoints of each. Meme ... etc. was used to identify shared patterns between the deletions and compare these were previously studies on non-recurrent deletions. Results: We discovered two novel sequence motifs shared between all 17 NRXN1 deletions and a significantly higher AT nucleotide content at the breakpoints, compared to the overall nucleotide content on chromosome 2. We found different alteration of sequence at the breakpoint; small insertions and duplications giving rise to short microhomology sequences. Conclusions: No single mechanism seems to be implicated in the deletion events, but the results suggest that NHEJ, FoSTeS or MMBIR is implicated. The two novel sequence motifs together with a high AT content in all in NRXN1 deletions may lead to increased instability leading to a increase susceptibility to a single stranded structures. This favours potentially repaired by NHEJ mechanism of double strand breaks or may leading to replication errors.

AB - Background: Genome instability plays fundamental roles in human evolution and phenotypic variation within our population. This instability leads to genomic rearrangements that are involved in a wide variety of human disorders, including congenital and neurodevelopmental disorders, and cancers. Insight into the molecular mechanisms governing such genomic rearrangements may increase our understanding of disease pathology and evolutionary processes. Here we analyse 17 carriers of non-recurrent deletions in the NRXN1 gene, which have been associated with neurodevelopmental disorders, e.g. schizophrenia, autism and epilepsies. Methods: 17 non-recurrent NRXN1 deletions identified by GWA were sequenced to map the breakpoints of each. Meme ... etc. was used to identify shared patterns between the deletions and compare these were previously studies on non-recurrent deletions. Results: We discovered two novel sequence motifs shared between all 17 NRXN1 deletions and a significantly higher AT nucleotide content at the breakpoints, compared to the overall nucleotide content on chromosome 2. We found different alteration of sequence at the breakpoint; small insertions and duplications giving rise to short microhomology sequences. Conclusions: No single mechanism seems to be implicated in the deletion events, but the results suggest that NHEJ, FoSTeS or MMBIR is implicated. The two novel sequence motifs together with a high AT content in all in NRXN1 deletions may lead to increased instability leading to a increase susceptibility to a single stranded structures. This favours potentially repaired by NHEJ mechanism of double strand breaks or may leading to replication errors.

U2 - 10.1002/ajmg.b.32204

DO - 10.1002/ajmg.b.32204

M3 - Journal article

C2 - 24339137

SN - 1552-4841

VL - 165

SP - 52

EP - 61

JO - American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics

JF - American Journal of Medical Genetics, Part B: Neuropsychiatric Genetics

IS - 1

ER -