TY - JOUR
T1 - Selecting Schizosaccharomyces pombe diploids
AU - Ekwall, Karl
AU - Thon, Genevieve
PY - 2017/7
Y1 - 2017/7
N2 - Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an h− strain with an h90 mat2-Pi-102 strain can be used to prevent sporulation. When ade6-M210/ade6-M216 mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.
AB - Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these cases mating an h− strain with an h90 mat2-Pi-102 strain can be used to prevent sporulation. When ade6-M210/ade6-M216 mutations impact on, or show synthetic interactions with, the gene of interest, two different auxotrophic markers can be used to select complementation.
UR - http://www.scopus.com/inward/record.url?scp=85021729779&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot091702
DO - 10.1101/pdb.prot091702
M3 - Journal article
C2 - 28679702
AN - SCOPUS:85021729779
SN - 1940-3402
VL - 2017
SP - 537
EP - 540
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 7
ER -