TY - JOUR
T1 - Screening and quantitative determination of twelve acidic and neutral pharmaceuticals in whole blood by liquid-liquid extraction and liquid chromatography-tandem mass spectrometry
AU - Simonsen, Kirsten Wiese
AU - Steentoft, Anni
AU - Buck, Maike
AU - Hansen, Lene
AU - Linnet, Kristian
PY - 2010/9
Y1 - 2010/9
N2 - We describe a multi-method for simultaneous identification and quantification of 12 acidic and neutral compounds in whole blood. The method involves a simple liquid-liquid extraction, and the identification and quantification are performed using liquid chromatography-tandem mass spectrometry. The method was fully validated for salicylic acid, paracetamol, phenobarbital, carisoprodol, meprobamate, topiramate, etodolac, chlorzoxazone, furosemide, ibuprofen, warfarin, and salicylamide. The method also tentatively includes thiopental, theophylline, piroxicam, naproxen, diclophenac, and modafinil, but these drugs were not included in the full validation program and are not described in detail here. Limit of quantitation was 1 mg/kg for the compounds with coefficients of variation of < 20%, except for furosemide, which had a coefficient of variation of 32% at limit of quantitation. The measuring interval was wide for most components. Extraction efficiencies were high, reflecting the high-yield capacity of the method.
AB - We describe a multi-method for simultaneous identification and quantification of 12 acidic and neutral compounds in whole blood. The method involves a simple liquid-liquid extraction, and the identification and quantification are performed using liquid chromatography-tandem mass spectrometry. The method was fully validated for salicylic acid, paracetamol, phenobarbital, carisoprodol, meprobamate, topiramate, etodolac, chlorzoxazone, furosemide, ibuprofen, warfarin, and salicylamide. The method also tentatively includes thiopental, theophylline, piroxicam, naproxen, diclophenac, and modafinil, but these drugs were not included in the full validation program and are not described in detail here. Limit of quantitation was 1 mg/kg for the compounds with coefficients of variation of < 20%, except for furosemide, which had a coefficient of variation of 32% at limit of quantitation. The measuring interval was wide for most components. Extraction efficiencies were high, reflecting the high-yield capacity of the method.
KW - Blood Chemical Analysis
KW - Chemical Fractionation
KW - Chromatography, Liquid
KW - Forensic Medicine
KW - Humans
KW - Hydrogen-Ion Concentration
KW - Pharmaceutical Preparations
KW - Reproducibility of Results
KW - Sensitivity and Specificity
KW - Substance Abuse Detection
KW - Tandem Mass Spectrometry
M3 - Journal article
C2 - 20822673
SN - 0146-4760
VL - 34
SP - 367
EP - 373
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
IS - 7
ER -
