Role of the chloroplast in the predatory dinoflagellate Karlodinium armiger

TY - JOUR

T1 - Role of the chloroplast in the predatory dinoflagellate Karlodinium armiger

AU - Berge, Terje

AU - Hansen, Per Juel

PY - 2016/5/10

Y1 - 2016/5/10

N2 - Karlodinium armiger is a phagotrophic dinoflagellate that synthesizes several small chloroplasts of haptophyte origin. It depends on light, but it grows very poorly in standard nutrient growth media (f/2) without food. When fed prey in the light, growth rates increase dramatically (μ = 0.65 d-1), suggesting that it relies heavily on phagotrophic nutrition. To explore the reasons for this phenomenon and the role of the plastids for the growth of K. armiger, we measured C14 fixation rates, chl a content and growth rates as a function of irradiance in short-and long-term preystarved cultures. In starved cultures, rates of photosynthesis were relatively high and increased as a function of irradiance, even though growth rates remained low. In fed cultures, both ingestion and growth rates of K. armiger increased as a function of irradiance in a saturating manner. Cellular chl a contents and photosynthetic rates were ∼40 and ∼70% lower, respectively, in longterm compared to short-term starved phototrophic cells at irradiances of 70 to 250 μmol photons m-2 s-1. Carbon fixation was important for achieving high mixotrophic growth rates, especially in prey-limited conditions. However, under prey-saturated conditions, K. armiger seems to obtain most of its carbon through phagotrophy. Our data suggest that feeding stimulates the photo-synthetic machinery under nutrient limitation, and that the poor phototrophic growth capacity of K. armiger in the absence of prey seems to be due to a lack or a very poor ability to take up essential inorganic nutrients.

AB - Karlodinium armiger is a phagotrophic dinoflagellate that synthesizes several small chloroplasts of haptophyte origin. It depends on light, but it grows very poorly in standard nutrient growth media (f/2) without food. When fed prey in the light, growth rates increase dramatically (μ = 0.65 d-1), suggesting that it relies heavily on phagotrophic nutrition. To explore the reasons for this phenomenon and the role of the plastids for the growth of K. armiger, we measured C14 fixation rates, chl a content and growth rates as a function of irradiance in short-and long-term preystarved cultures. In starved cultures, rates of photosynthesis were relatively high and increased as a function of irradiance, even though growth rates remained low. In fed cultures, both ingestion and growth rates of K. armiger increased as a function of irradiance in a saturating manner. Cellular chl a contents and photosynthetic rates were ∼40 and ∼70% lower, respectively, in longterm compared to short-term starved phototrophic cells at irradiances of 70 to 250 μmol photons m-2 s-1. Carbon fixation was important for achieving high mixotrophic growth rates, especially in prey-limited conditions. However, under prey-saturated conditions, K. armiger seems to obtain most of its carbon through phagotrophy. Our data suggest that feeding stimulates the photo-synthetic machinery under nutrient limitation, and that the poor phototrophic growth capacity of K. armiger in the absence of prey seems to be due to a lack or a very poor ability to take up essential inorganic nutrients.

U2 - 10.3354/meps11682

DO - 10.3354/meps11682

M3 - Journal article

SN - 0171-8630

VL - 549

SP - 41

EP - 54

JO - Marine Ecology - Progress Series

JF - Marine Ecology - Progress Series

ER -