TY - JOUR
T1 - Role of SeqA and Dam in Escherichia coli gene expression
T2 - a global/microarray analysis
AU - Løbner-Olesen, Anders
AU - Marinus, Martin G
AU - Hansen, Flemming G
PY - 2003/4/15
Y1 - 2003/4/15
N2 - High-density oligonucleotide arrays were used to monitor global transcription patterns in Escherichia coli with various levels of Dam and SeqA proteins. Cells lacking Dam methyltransferase showed a modest increase in transcription of the genes belonging to the SOS regulon. Bacteria devoid of the SeqA protein, which preferentially binds hemimethylated DNA, were found to have a transcriptional profile almost identical to WT bacteria overexpressing Dam methyltransferase. The latter two strains differed from WT in two ways. First, the origin proximal genes were transcribed with increased frequency due to increased gene dosage. Second, chromosomal domains of high transcriptional activity alternate with regions of low activity, and our results indicate that the activity in each domain is modulated in the same way by SeqA deficiency or Dam overproduction. We suggest that the methylation status of the cell is an important factor in forming and/or maintaining chromosome structure.
AB - High-density oligonucleotide arrays were used to monitor global transcription patterns in Escherichia coli with various levels of Dam and SeqA proteins. Cells lacking Dam methyltransferase showed a modest increase in transcription of the genes belonging to the SOS regulon. Bacteria devoid of the SeqA protein, which preferentially binds hemimethylated DNA, were found to have a transcriptional profile almost identical to WT bacteria overexpressing Dam methyltransferase. The latter two strains differed from WT in two ways. First, the origin proximal genes were transcribed with increased frequency due to increased gene dosage. Second, chromosomal domains of high transcriptional activity alternate with regions of low activity, and our results indicate that the activity in each domain is modulated in the same way by SeqA deficiency or Dam overproduction. We suggest that the methylation status of the cell is an important factor in forming and/or maintaining chromosome structure.
KW - Bacterial Outer Membrane Proteins
KW - Chromosomes, Bacterial/genetics
KW - DNA Methylation
KW - DNA, Bacterial/genetics
KW - DNA-Binding Proteins
KW - Escherichia coli/genetics
KW - Escherichia coli Proteins/genetics
KW - Gene Expression Regulation, Bacterial
KW - Genes, Bacterial
KW - Oligonucleotide Array Sequence Analysis
KW - SOS Response (Genetics)
KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics
KW - Transcription Factors/genetics
U2 - 10.1073/pnas.0538053100
DO - 10.1073/pnas.0538053100
M3 - Journal article
C2 - 12682301
SN - 0027-8424
VL - 100
SP - 4672
EP - 4677
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -