RNA-Seq for Bacterial Gene Expression

TY - JOUR

T1 - RNA-Seq for Bacterial Gene Expression

AU - Poulsen, Line Dahl

AU - Vinther, Jeppe

PY - 2018

Y1 - 2018

N2 - RNA sequencing (RNA-seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor-intensive and expensive part of an RNA-seq experiment is the preparation of sequencing libraries, which is also essential for the quality of the data obtained. Here, we present a straightforward and inexpensive basic protocol for preparation of strand-specific RNA-seq libraries from bacterial RNA as well as a computational pipeline for the data analysis of sequencing reads. The protocol is based on the Illumina platform and allows easy multiplexing of samples and the removal of sequencing reads that are PCR duplicates.

AB - RNA sequencing (RNA-seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor-intensive and expensive part of an RNA-seq experiment is the preparation of sequencing libraries, which is also essential for the quality of the data obtained. Here, we present a straightforward and inexpensive basic protocol for preparation of strand-specific RNA-seq libraries from bacterial RNA as well as a computational pipeline for the data analysis of sequencing reads. The protocol is based on the Illumina platform and allows easy multiplexing of samples and the removal of sequencing reads that are PCR duplicates.

KW - library construction

KW - next-generation sequencing

KW - RNA-seq

U2 - 10.1002/cpnc.55

DO - 10.1002/cpnc.55

M3 - Journal article

C2 - 29927111

AN - SCOPUS:85054136058

SN - 1934-9270

VL - 73

SP - 1

EP - 12

JO - Current Protocols in Nucleic Acid Chemistry

JF - Current Protocols in Nucleic Acid Chemistry

IS - 1

M1 - e55

ER -