RNA Decay by Messenger RNA Interferases

Mikkel Christensen-Dalsgaard; Martin Overgaard; Kristoffer Skovbo Winther; Kenn Gerdes

doi:10.1016/S0076-6879(08)02225-8

RNA Decay by Messenger RNA Interferases

Mikkel Christensen-Dalsgaard, Martin Overgaard, Kristoffer Skovbo Winther, Kenn Gerdes

24 Citationer (Scopus)

Abstract

Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and the corresponding antitoxin ROB. In particular, we describe a set of plasmid vectors useful for the detailed analysis of cleavage sites in model mRNAs.

Originalsprog	Engelsk
Bogserie	Methods in Enzymology
Vol/bind	447
Sider (fra-til)	521-535
Antal sider	15
ISSN	0076-6879
DOI	https://doi.org/10.1016/S0076-6879(08)02225-8
Status	Udgivet - 2008
Udgivet eksternt	Ja

Adgang til dokumentet

10.1016/S0076-6879(08)02225-8

Citationsformater

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title = "RNA Decay by Messenger RNA Interferases",

abstract = "Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and the corresponding antitoxin ROB. In particular, we describe a set of plasmid vectors useful for the detailed analysis of cleavage sites in model mRNAs.",

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AU - Overgaard, Martin

AU - Winther, Kristoffer Skovbo

AU - Gerdes, Kenn

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N2 - Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and the corresponding antitoxin ROB. In particular, we describe a set of plasmid vectors useful for the detailed analysis of cleavage sites in model mRNAs.

AB - Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and the corresponding antitoxin ROB. In particular, we describe a set of plasmid vectors useful for the detailed analysis of cleavage sites in model mRNAs.

U2 - 10.1016/S0076-6879(08)02225-8

DO - 10.1016/S0076-6879(08)02225-8

M3 - Journal article

SN - 0076-6879

VL - 447

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EP - 535

JO - Methods in Enzymology

JF - Methods in Enzymology

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