TY - JOUR
T1 - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
AU - Xie, Yakun
AU - Huhn, Kerstin
AU - Brandt, Ronny
AU - Potschin, Maren
AU - Bieker, Stefan
AU - Straub, Daniel
AU - Doll, Jasmin
AU - Drechsler, Thomas
AU - Zentgraf, Ulrike
AU - Wenkel, Stephan
N1 - © 2014. Published by The Company of Biologists Ltd.
PY - 2014/12/15
Y1 - 2014/12/15
N2 - As sessile organisms, plants have to continuously adjust growth and development to ever-changing environmental conditions. At the end of the growing season, annual plants induce leaf senescence to reallocate nutrients and energy-rich substances from the leaves to the maturing seeds. Thus, leaf senescence is a means with which to increase reproductive success and is therefore tightly coupled to the developmental age of the plant. However, senescence can also be induced in response to sub-optimal growth conditions as an exit strategy, which is accompanied by severely reduced yield. Here, we show that class III homeodomain leucine zipper (HD-ZIPIII) transcription factors, which are known to be involved in basic pattern formation, have an additional role in controlling the onset of leaf senescence in Arabidopsis. Several potential direct downstream genes of the HD-ZIPIII protein REVOLUTA (REV) have known roles in environment-controlled physiological processes. We report that REV acts as a redox-sensitive transcription factor, and directly and positively regulates the expression of WRKY53, a master regulator of age-induced leaf senescence. HD-ZIPIII proteins are required for the full induction of WRKY53 in response to oxidative stress, and mutations in HD-ZIPIII genes strongly delay the onset of senescence. Thus, a crosstalk between early and late stages of leaf development appears to contribute to reproductive success.
AB - As sessile organisms, plants have to continuously adjust growth and development to ever-changing environmental conditions. At the end of the growing season, annual plants induce leaf senescence to reallocate nutrients and energy-rich substances from the leaves to the maturing seeds. Thus, leaf senescence is a means with which to increase reproductive success and is therefore tightly coupled to the developmental age of the plant. However, senescence can also be induced in response to sub-optimal growth conditions as an exit strategy, which is accompanied by severely reduced yield. Here, we show that class III homeodomain leucine zipper (HD-ZIPIII) transcription factors, which are known to be involved in basic pattern formation, have an additional role in controlling the onset of leaf senescence in Arabidopsis. Several potential direct downstream genes of the HD-ZIPIII protein REVOLUTA (REV) have known roles in environment-controlled physiological processes. We report that REV acts as a redox-sensitive transcription factor, and directly and positively regulates the expression of WRKY53, a master regulator of age-induced leaf senescence. HD-ZIPIII proteins are required for the full induction of WRKY53 in response to oxidative stress, and mutations in HD-ZIPIII genes strongly delay the onset of senescence. Thus, a crosstalk between early and late stages of leaf development appears to contribute to reproductive success.
KW - Alcohol Oxidoreductases
KW - Arabidopsis
KW - Arabidopsis Proteins
KW - Chromatin Immunoprecipitation
KW - Cysteine Endopeptidases
KW - DNA-Binding Proteins
KW - Gene Expression Regulation, Plant
KW - Homeodomain Proteins
KW - Hydrogen Peroxide
KW - Leucine Zippers
KW - Plant Leaves
KW - Real-Time Polymerase Chain Reaction
KW - Transcription Factors
U2 - 10.1242/dev.117689
DO - 10.1242/dev.117689
M3 - Journal article
C2 - 25395454
SN - 0950-1991
VL - 141
SP - 4772
EP - 4783
JO - Development
JF - Development
IS - 24
ER -