Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer

TY - JOUR

T1 - Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer

AU - van de Weert, Marco

AU - van Dijkhuizen-Radersma, Riemke

AU - Bezemer, Jeroen M

AU - Hennink, Wim E

AU - Crommelin, Daan J A

PY - 2002/7

Y1 - 2002/7

N2 - Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.

AB - Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.

M3 - Journal article

C2 - 12084507

SN - 1873-3441

VL - 54

SP - 89

EP - 93

JO - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

IS - 1

ER -