Restoration of growth by manganese in a mutant strain of Escherichia coli lacking most known iron and manganese uptake systems

TY - JOUR

T1 - Restoration of growth by manganese in a mutant strain of Escherichia coli lacking most known iron and manganese uptake systems

AU - Taudte, Nadine

AU - German, Nadezhda

AU - Zhu, Yong-Guan

AU - Grass, Gregor

AU - Rensing, Christopher Günther T

PY - 2016/6/1

Y1 - 2016/6/1

N2 - The interplay of manganese and iron homeostasis and oxidative stress in Escherichia coli can give important insights into survival of bacteria in the phagosome and under differing iron or manganese bioavailabilities. Here, we characterized a mutant strain devoid of all know iron/manganese-uptake systems relevant for growth in defined medium. Based on these results an exit strategy enabling the cell to cope with iron depletion and use of manganese as an alternative for iron could be shown. Such a strategy would also explain why E. coli harbors some iron- or manganese-dependent iso-enzymes such as superoxide dismutases or ribonucleotide reductases. The benefits for gaining a means for survival would be bought with the cost of less efficient metabolism as indicated in our experiments by lower cell densities with manganese than with iron. In addition, this strain was extremely sensitive to the metalloid gallium but this gallium toxicity can be alleviated by low concentrations of manganese.

AB - The interplay of manganese and iron homeostasis and oxidative stress in Escherichia coli can give important insights into survival of bacteria in the phagosome and under differing iron or manganese bioavailabilities. Here, we characterized a mutant strain devoid of all know iron/manganese-uptake systems relevant for growth in defined medium. Based on these results an exit strategy enabling the cell to cope with iron depletion and use of manganese as an alternative for iron could be shown. Such a strategy would also explain why E. coli harbors some iron- or manganese-dependent iso-enzymes such as superoxide dismutases or ribonucleotide reductases. The benefits for gaining a means for survival would be bought with the cost of less efficient metabolism as indicated in our experiments by lower cell densities with manganese than with iron. In addition, this strain was extremely sensitive to the metalloid gallium but this gallium toxicity can be alleviated by low concentrations of manganese.

KW - Journal Article

U2 - 10.1007/s10534-016-9927-3

DO - 10.1007/s10534-016-9927-3

M3 - Journal article

C2 - 27003826

SN - 0966-0844

VL - 29

SP - 433

EP - 450

JO - BioMetals

JF - BioMetals

IS - 3

ER -