Response of the bacterial community in an on-farm biopurification system, to which diverse pesticides are introduced over an agricultural season

TY - JOUR

T1 - Response of the bacterial community in an on-farm biopurification system, to which diverse pesticides are introduced over an agricultural season

AU - Holmsgaard, Peter Nikolai

AU - Dealtry, Simone

AU - Dunon, Vincent

AU - Heuer, Holger

AU - Hansen, Lars H.

AU - Springael, Dirk

AU - Smalla, Kornelia

AU - Riber, Leise

AU - Sørensen, Søren Johannes

PY - 2017/10

Y1 - 2017/10

N2 - A biopurification system (BPS) is used on-farm to clean pesticide-contaminated wastewater. Due to high pesticide loads, a BPS represents a hot spot for the proliferation and selection as well as the genetic adaptation of discrete pesticide degrading microorganisms. However, while considerable knowledge exists on the biodegradation of specific pesticides in BPSs, the bacterial community composition of these systems has hardly been explored. In this work, the Shannon diversity, the richness and the composition of the bacterial community within an operational BPS receiving wastewater contaminated with various pesticides was, for the first time, elucidated over the course of an agricultural season, using DGGE profiling and pyrosequencing of 16S rRNA gene fragments amplified from total community DNA. During the agricultural season, an increase in the concentration of pesticides in the BPS was observed along with the detection of significant community changes including a decrease in microbial diversity. Additionally, a significant increase in the relative abundance of Proteobacteria, mainly the Gammaproteobacteria, was found, and OTUs (operational taxonomic units) affiliated to Pseudomonas responded positively during the course of the season. Furthermore, a banding-pattern analysis of 16S rRNA gene-based DGGE fingerprinting, targeting the Alpha- and Betaproteobacteria as well as the Actinobacteria, indicated that the Betaproteobacteria might play an important role. Interestingly, a decrease of Firmicutes and Bacteroidetes was observed, indicating their selective disadvantage in a BPS, to which pesticides have been introduced.

AB - A biopurification system (BPS) is used on-farm to clean pesticide-contaminated wastewater. Due to high pesticide loads, a BPS represents a hot spot for the proliferation and selection as well as the genetic adaptation of discrete pesticide degrading microorganisms. However, while considerable knowledge exists on the biodegradation of specific pesticides in BPSs, the bacterial community composition of these systems has hardly been explored. In this work, the Shannon diversity, the richness and the composition of the bacterial community within an operational BPS receiving wastewater contaminated with various pesticides was, for the first time, elucidated over the course of an agricultural season, using DGGE profiling and pyrosequencing of 16S rRNA gene fragments amplified from total community DNA. During the agricultural season, an increase in the concentration of pesticides in the BPS was observed along with the detection of significant community changes including a decrease in microbial diversity. Additionally, a significant increase in the relative abundance of Proteobacteria, mainly the Gammaproteobacteria, was found, and OTUs (operational taxonomic units) affiliated to Pseudomonas responded positively during the course of the season. Furthermore, a banding-pattern analysis of 16S rRNA gene-based DGGE fingerprinting, targeting the Alpha- and Betaproteobacteria as well as the Actinobacteria, indicated that the Betaproteobacteria might play an important role. Interestingly, a decrease of Firmicutes and Bacteroidetes was observed, indicating their selective disadvantage in a BPS, to which pesticides have been introduced.

KW - 16S rRNA gene sequencing

KW - Bacterial communities

KW - Biopurification system

KW - DGGE profiling

KW - Pesticide degradation

KW - Shannon diversity

UR - http://www.scopus.com/inward/record.url?scp=85025481590&partnerID=8YFLogxK

U2 - 10.1016/j.envpol.2017.07.026

DO - 10.1016/j.envpol.2017.07.026

M3 - Journal article

C2 - 28734695

AN - SCOPUS:85025481590

SN - 0269-7491

VL - 229

SP - 854

EP - 862

JO - Environmental Pollution

JF - Environmental Pollution

ER -